| 田燕燕 杨烨 张园园 俞睿 朱佳 肖拉提 朱筠.降低TGFβ1 表达后对大鼠糖尿病症状改变的研究[J].,2014,14(27):5217-5219 |
| 降低TGFβ1 表达后对大鼠糖尿病症状改变的研究 |
| The Research of Knock down TGFβ1 Expression Altered DiabetesSymptoms Change |
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| DOI： |
| 中文关键词: TGFβ1 慢病毒 糖尿病 |
| 英文关键词: TGFβ1 Lentiviral Vectors Diabetes |
| 基金项目:国家自然科学基金项目（81160116） |
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| 中文摘要: |
| 目的：探讨降低TGFβ1 的表达后，对大鼠糖尿病模型病症的改变情况，为研究TGFβ1 与糖尿病发病之间提供实验数据。方法：采用腹腔注射链脲佐菌素，建立糖尿病大鼠模型，慢病毒包装TGFβ1-shRNA干扰载体，优选后皮下注射大鼠模型，以慢病毒空载shRNA注射大鼠模型作对照；检测1-5 周左右两组大鼠体重和血糖变化；摘取大鼠胰腺，HE 染色光学镜下观察组织形态学改变情况。结果：用RNAi沉默TGFβ1 表达后，与对照组相比，干扰组大鼠体重下降较缓，血糖持续下降；HE 染色可见干扰组大鼠
胰岛形态较空载组稍好，分泌细胞数量稍多。结论：TGFβ1 对糖尿病的发病和进展起促进作用，当抑制TGF茁1 表达后，可延缓糖
尿病的进展。 |
| 英文摘要: |
| Objective:To research the changes of SD rat Diabetes symptoms via knocking down TGFβ1 expression, to observe
the relationship between TGFβ1 and Diabetes process.Methods:Intraperitoneal injection of drug STZ was used to set up Diabetic SD rat
model, Packaging TGFβ1-shRNA lentiviral vectors were injected subcutaneously into SD rat model while shRNA was injected in control
groups. the change of SD rat body weight and blood glucose levels were detected.Results:After TGFβ1 were silenced by RNAi,
compared with the control group, the disturbance of SD rat weight loss was slower, blood sugar levels continued to decline; HE dyed
showed that in interference group, SD rat pancreas islet had a slightly better morphology, presenting slightly more secretory cells.Conclusion:TGFβ1 was a stimulus to the onset and progress of diabetes, it can delay the progress of diabetes after the inhibition of the
expression of TGF beta 1. |
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