| 刘春富 李卫 许军 翟博 宫金伟.乳腺癌组织中转化生长因子-beta1 和血管内皮生长因子的表达
及其与血管生成的关系[J].,2014,14(26):5104-5108 |
| 乳腺癌组织中转化生长因子-beta1 和血管内皮生长因子的表达
及其与血管生成的关系 |
| Expression of Transforming Growth factor-beta1 and Vascular Endothelial CellGrowth Factor and Their Correlation with Angiogenesi in the Breast Cancer |
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| DOI： |
| 中文关键词: 乳腺癌 血管内皮生长因子 转化生长因子-beta1 微血管密度 |
| 英文关键词: Breast cancer Vascular endothelial cell growth factor (VEGF) Transforming growth factor-beta1 (TGF-beta1) Microvessel
density(MVD) |
| 基金项目:黑龙江省科技创新资助项目（GC09C407-4） |
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| 中文摘要: |
| 目的：研究转化生长因子-beta1(transforming growth factor-beta1，TGF-beta1)和血管内皮生长因子(vascular endothelial cell growth
factor，VEGF)在乳腺癌组织中的表达及其与血管生成的关系。方法：选取65 例手术切除乳腺癌蜡块标本及其周围正常乳腺组织，分为两组：A 组为对照组，检测标本为乳腺癌癌旁正常乳腺组织；B 组为实验组，检测标本为乳腺癌组织，采用免疫组织化学染色和形态计量检测TGF-beta1 和VEGF 在乳腺癌组织中的表达。利用CD34 相关抗原标记血管内皮细胞，计数微血管密度(intratumoral mier oveseulardensity，MVD)，并分析其与TGF-beta1 和VEGF表达的关系。结果：65 例乳腺癌组织中，TGF-beta1 的阳性
表达率为69.23%(45/65)，TGF-beta1 阳性表达者MVD 值(25.31± 4.05)显著高于TGF-beta1 阴性表达者(21.23± 4.29)；VEGF的阳性表达率为78.46%(51/65)，VEGF阳性表达者MVD值(26.62± 3.41)亦明显显著高于VEGF阴性表达者(18.95± 6.52)(均P<0.05)。不同病理类型的乳腺癌组织中TGF-beta1、VEGF的阳性表达率比较差异无统计学意义(P>0.05)，但TGF-beta1、VEGF的阳性表达与乳腺癌的组织分级、淋巴结转移呈显著正相关(均P<0.05)，且组织学分级越高、淋巴结转移越多，MVD值越大。结论：TGF-beta1 与VEGF在乳腺癌组织的表达高于正常乳腺组织，并与乳腺癌肿瘤血管的生成有关，二者有望作为乳腺癌恶性程度、浸润转移等生物学行为的评估指标。 |
| 英文摘要: |
| Objective:To investigate the expression of transforming growth factor-beta1 (TGF-beta1) and vascular endothelial cell
growth factor(VEGF) and their correlation with angiogenesi in the breast cancer.Methods:65 cases of operation excision of breast cancer
paraffin blocks and the surrounding normal breast tissue were selected and divided into two groups: group A was the control group, the
specimens of which were breast cancer adjacent normal breast tissue; group B was the experimental group, the specimens of which were
breast cancer tissue. The expression of TGF -beta1 and VEGF were detected by immunohistochemical staining and morphometric study.
Micro-vessel density (MVD) were tested by CD34 related antigen as marker of vascular endothelial cell and the correlation of MVD with
expression of TGF-beta1 and VEGF were analyzed.Results:In 65 cases of breast cancer, the positive expression rate of TGF-beta1 was
69.23%(45/65), the MVD value of those TGF-beta1 positive expression(26.18± 4.05) was significantly higher than that in TGF-beta1 negative
expression(20.13± 4.29). The positive expression rate of VEGF was 78.46%(51/65), the MVD value of those VEGF positive expression
(25.82± 3.61) was also significantly higher than that in the negative group (19.65± 6.32) (P<0.05). The positive expression rate of VEGF
and TGF-beta1 showed no statistical difference in different pathological types of breast cancer tissues (P>0.05). However, according to the
histological grade, lymph node metastasis showed significant correlation, and the higher histological lymph node metastasis was, the
higher MVD value was.Conclusion:The expression of TGF-beta1 and VEGF in breast cancer tissue were higher than those in the adjacent
normal breast tissue, and were correlated with the angiogenesis in the breast cancer. TGF-beta1 and VEGF could be used as the evaluation
index of biological behavior such as malignant degree, invasion, metastasis of breast cancer. |
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