| 段岩 刘新宇 李凤华 惠丽超 王硕 张晓妍.胰高血糖素样肽l对脂多糖诱导的血管内皮细胞炎性反应的影响[J].,2014,14(24):4652-4655 |
| 胰高血糖素样肽l对脂多糖诱导的血管内皮细胞炎性反应的影响 |
| Effect of Glucagon Like Peptide-1 on Lipopolysaccharide-inducedInflammatory Response in the Vascular Endothelial Cells |
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| DOI： |
| 中文关键词: 胰高血糖素样肽-l 血管内皮细胞 炎性反应 脂多糖 |
| 英文关键词: Glucagon like peptide-1(GLP-1) Vascular endothelial cells(VEC) Inflammatory response Lipopolysaccharide |
| 基金项目:辽宁省优秀硕士论文课题（2012071） |
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| 中文摘要: |
| 目的：探讨胰高血糖素样肽l (glucagon like peptide 1，GLP-1)对脂多糖(1ipopolysaccharide，LPS)诱导的血管内皮细胞(VEC)
炎性反应的影响。方法：以体外培养的人动脉VEC为研究模型，将细胞分为四组(对照组、LPS 刺激组、LPS+GLP-1 组、GLP-1 组)，
Rhodamin-Phalloidin 检测肌动蛋白骨架F-actin 分布，用苏木素-伊红(HE)染色观察细胞间连接的形态特征，用示踪剂Rhodamine
B isothiocyanate-Dextran 检测VECs 单层通透性变化改变，酶联免疫吸附实验检测细胞分泌白介素(IL)-6 和IL-8 的变化。结果：GLP-1(100 nM)可减少LPS(1 ug/mL)刺激后细胞肌动蛋白骨架F-actin 应力纤维的形成，并抑制LPS 刺激后细胞间连接的中断。Rhodamine B isothiocyanate-Dextran 细胞通透性检测结果显示：GLP-1 可明显降低LPS 刺激引起的VEC通透性增加[由(2.57±0.19)× 10-5cm/s降至(2.10± 0.18)× 10-5 cm/s，P<0.05]。此外，GLP-1 可抑制LPS 刺激后VEC中炎性细胞因子IL-6 和IL-8 的表达[分别由(42130± 6522)pg/ml降至(27478± 5096)pg/ml 和(18376± 1561)pg/ml 降至(14414 ± 927)pg/ml，均P<0.05]。结论：GLP-1 可对抗LPS刺激引起的VEC炎症反应和细胞通透性增加，改善LPS诱导的内皮细胞炎性损伤。 |
| 英文摘要: |
| Objective:To investigate the effect of glucagon like peptide-1 (GLP-1) on 1ipopolysaccharide (LPS)-induced
inflammatory response in the vascular endothelial cells (VECs).Methods:In vitro cultured human artery VECs were exposed to four
groups (control, LPS, LPS + GLP-1 and GLP-1). Distribution of cell skeletal protein filamentous actin (F-actin) was detected by
Rhodamin-Phalloidin staining. Hematoxylin-eosin staining was used to observe the morphological features of intercellular connections.
Endothelial permeability was detected by measuring the flux of Rhodamine B isothiocyanate-Dextran across the VEC monolayers.
Interleukin (IL)-6 and IL-8 secretion fromcells was determined by using enzyme immunolinked assay (ELISA).Results:GLP-1 (100 nM)
attenuated the formation of F-action stress fiber induced by LPS (1 ug/mL) and inhibited the dissociation of intercellular connections after LPS exposure. Cell permeability test using Rhodamine B isothiocyanate-Dextran indicated that GLP-1 effectively alleviated the hyperpermeability of VEC monolayer from (2.57 ± 0.19) × 10-5 cm/s to (2.10 ± 0.18) × 10-5 cm/s (P < 0.05). In addition, GLP-1
inhibited the secretion of inflammatory cytokines IL-6 and IL-8 induced by LPS [from (42130 ± 6522) pg/ml to (27478 ± 5096) pg/ml
and (18376 ± 1561) pg/ml to (14414 ± 927) pg/ml, respectively, both P < 0.05].Conclusion:GLP-1 could combat LPS-induced
inflammatory responses and the hyperpermeability of VECs, and thereby antagonize LPS-induced pathological cellular injury. |
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