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目的：研究福尔马林致痛后大鼠脊髓和背根神经节（dorsal root ganglion, DRG）内降钙素基因相关肽（calcitonin gene-related peptide，CGRP）的时空变化规律，为探讨CGRP 在伤害性信息传递中的机制和作用提供实验依据。方法：选取健康成年正常SD 大鼠54 只随机分为生理盐水对照组和福尔马林实验组；实验组为右侧足底皮下给予0.1 mL 5%福尔马林后，分别存活15 min、30 min、1 h、3 h、6 h、12h、24h 和72h (n=6)，免疫组化结合图像分析技术观测CGRP 在脊髓腰段及L4~6DRG 的表达变化。结果：正常 DRG、脊髓前角和后角内CGRP 有一定的基础表达。福尔马林致痛后3 h DRG的CGRP 表达上升，12 h～24 h达峰值，72 h 基本降低到正常；福尔马林致痛后6 h 脊髓后角CGRP 的表达上调，24 h达到高峰，72 h 降低至正常；脊髓前角CGRP 未见明显变化。结论：福尔马林致痛引起DRG和脊髓的CGRP 的表达呈现一定的时空模式，可能是其参与伤害性信息传递的机制之一。

英文摘要:

Objective:To observe the temporospatial expression changing laws of calcitonin gene-related peptide (CGRP) in the spinal cord and dorsal root ganglion (DRG) after subcutaneouly formalin injection in rats hind planta.Methods:54 healthy adult SD rats were distributed into physiological saline group and experimental groups, while experimental groups were subdivided randomly into 15 min, 30 min, 1 h, 3 h, 6 h, 12 h, 24 h and 72 h groups (n=6) which were treated with 5%formalin 100 滋L injected into rat hind planta. The expression and distribution of CGRP in the lumber spinal cord and L4~6 DRG were detected by immunohistochemical method and image analysis technique.Results:CGRP was expressed in normal anterior and posterior horns of spinal cord and DRG. The expression of CGRP in DRG neurons was up-regulated in 3h group after subcutaneouly formalin injection. However, changes of CGRP in DRG peaked at 12 h～24 h groups, then decreased to normal level at 72 h group. The expression of CGRP increased in the lamina Ⅱ of spinal cord at 6 h group, and peaked at 12 h～24 h groups, then decreased to normal level at 72 h group. There were no changes in the CGRP-positive spinal ventral motoneurons of all groups.Conclusion:The changes of CGRP presented a temporospatial pattern in the spinal cord and DRG after formalin injection, which may be one of mechanisms that participated in the nociceptive signal transmission.

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