| 刘慰华 张竞之 刘少军 邱怀娜 郭景新 刘彬.黄芪含药血清对NK细胞活性及KLRK1 表达的影响[J].,2014,14(24):4637-4640 |
| 黄芪含药血清对NK细胞活性及KLRK1 表达的影响 |
| Effect of Radix Astragali Drug Serumon NK Cell Activity andExpression of KLRK1 |
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| DOI： |
| 中文关键词: 黄芪含药血清 NK 细胞 杀伤细胞凝集素样受体K1 |
| 英文关键词: Radix Astragali drug serum NK Cells Killer cell lectin-like receptor K1 |
| 基金项目:国家自然科学基金项目（81302892）；高等学校博士学科点专项科研基金（20124423120001）； |
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| 中文摘要: |
| 目的：探讨黄芪含药血清对自然杀伤（NK）细胞活性及杀伤细胞凝集素样受体K1（KLRK1）表达的影响。方法：SD大鼠灌胃
不同剂量的黄芪水煎剂制备黄芪含药血清。NK92MI细胞与不同剂量黄芪含药血清及对照血清孵育12 h后，采用乳酸脱氢酶释
放测定法检测NK 细胞对靶细胞YAC-1 的杀伤活性，采用qPCR和western blot 检测KLRK1 mRNA 和蛋白表达。结果：不同浓度
黄芪含药血清刺激12 h后NK 细胞杀伤活性明显增强，KLRK1 表达显著升高。结论：黄芪含药血清能活化NK 细胞，其机制可能
与其激活KLRK1 有关。 |
| 英文摘要: |
| Objective:To study the role of Radix Astragali drug serum on Natural killer (NK) cell activity and Killer cell
lectin-like receptor K1（KLRK1）expression.Methods:Radix Astragali drug serum was prepared on SD rats by gavage administration
with different doses of Radix Astragali. NK92MI cells were incubated with different doses of Radix Astragali drug serum and control
serumfor 12 hours. LDH release assay were used to determine the NK cells killing activity of target cells YAC-1. qPCR and western blot
were used to determine the mRNA and protein expression of Killer cell lectin-like receptor K1 (KLRK1), respectively.Results:After incubation
with different concentrations of Radix Astragali drug serum for 12 hours, the killing activity of NK cells and expression of KLRK1
increased significantly compared with control group.Conclusion:Radix Astragali drug serum can activate NK cells and underlying
mechanismmay be related to activate KLRK1. |
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