| 桑甜甜 曹青 王飞 黄黎亚 王玉强 陈书艳 刘芳 张建军.FOXO3a过表达对内皮祖细胞周期相关蛋白的调控[J].,2014,14(24):4626-4629 |
| FOXO3a过表达对内皮祖细胞周期相关蛋白的调控 |
| The Effects of FOXO3a Overexpression on the Cell Cycle RegulatoryProteins in Endothelial Progenitor Cells |
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| DOI： |
| 中文关键词: 内皮祖细胞 腺病毒载体 细胞增殖 |
| 英文关键词: Endothelial progenitor cells Adenovirus vector Cell proliferation |
| 基金项目:国家自然科学基金项目(30973152，8
1270205) |
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| 中文摘要: |
| 目的：观察FOXO3a（forkhead box O3a）的活性改变对内皮祖细胞（endothelial progenitor cells，EPCs）增殖和细胞周期相关蛋
白表达的影响。方法：将携带突变激活FOXO3a 基因的腺病毒载体Ad-TM (triple mutant)-FOXO3a 和阴性对照腺病毒载体
Ad-GFP 体外感染人脐血来源的EPCs。观察EPCs形态学改变，CCK-8 分析转染后EPCs增殖情况，Western blot检测FOXO3a 蛋
白、细胞周期相关蛋白p27kip1以及CDK2 的表达水平。结果：构建了的2 种腺病毒相关载体被成功转染。形态学改变方面，
Ad-TM-FOXO3a 组EPCs细胞生长缓慢，集落不明显；Western blot 和CCK-8 结果显示，Ad-TM-FOXO3a 转染组与阴性对照组相
比，EPCs 增殖被抑制，FOXO3a 与p27kip1蛋白过表达，CDK2 表达下调。结论：FOXO3a 可能通过上调p27kip1 蛋白表达，下调
CDK2 表达，以抑制EPCs增殖。 |
| 英文摘要: |
| Objective:To investigate the effects of FOXO3a overexpression on the proliferation of endothelial progenitor cells
(EPCs) and the expression of the cell cycle regulatory proteins.Methods:Two constructed recombinant adenovirus vectors, Ad-TM
(triple mutant)-FOXO3a and the control Ad-GFP, were transfected into EPCs from human cord blood in vitro. The changes in cell
morphology and proliferation of transfected EPCs were detected by fluorescent microscope and CCK-8 assay, respectively. The protein
expression changes of FOXO3a, cell cycle regulatory proteins p27kip1 and CDK2 were assessed by Western blot.Results:The both
recombinant adenovirus vectors were successfully transfected into EPCs. The results demonstrated that compared with control group, in
the Ad-TM-FOXO3a group had the overexpressed-FOXO3a, while cell division proceeded slowly and proliferation EPCs was
significantly inhibited; Meanwhile p27kip1 were upregulated while CDK2 expression was downregulated.Conclusion:FOXO3a may
inhibit proliferation of EPCs via up-regulating the expression of p27kip1 and down-regulating the expression of CDK2. |
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