| 王晓辉 龙润乡 张名 杨晓蕾 梁海孝 张云昆 邵聪文.HAV病毒液的3种浓缩方法比较[J].,2014,14(24):4616-4619 |
| HAV病毒液的3种浓缩方法比较 |
| The Comparison of Three Kinds of Virus Enrichment Methodsfor the HAV Virus |
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| DOI： |
| 中文关键词: HAV 超滤浓缩 回收率 |
| 英文关键词: HepatitisA virus Ultrafiltration concentrated Recovery rate |
| 基金项目:云南省应用基础研究面上项目（2010ZC230） |
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| 中文摘要: |
| 目的：对HAV 病毒液的3 种常见浓缩方法进行分析比较，为HAV病毒研究及规模化疫苗生产提供参考。方法：使用MILLIPORE
PELLICON 超滤、PEG 6000 沉淀、蔗糖- 甘油垫三种方法对纯化HAV 病毒液进行浓缩，用ELISA 方法对浓缩液进行抗
原滴度检测，计算不同浓缩方法的回收率。结果：HAV 病毒液经过7 次超滤循环浓缩，平均回收率为86 %；PEG 浓缩方法回收率
平均72.5 %；蔗糖- 甘油离心浓缩方法平均回收率53.3 %。结论：蔗糖/甘油超离心法，集纯化浓缩一体，适用于样品量较少，需要
高浓度样品的试验；PEG 浓缩得率适中，操作简单，应用范围较广；超滤膜浓缩在大规模疫苗生产或样品量较大时适用，但需控制
样品浓度及浓缩倍数不能太高，以免样品损失。 |
| 英文摘要: |
| Objective:Using three common concentrated methods to enrichment hepatitis A virus, and the refined effect is
analysed, concentrated compare different ways to use effect. Provide the reference for large-scale vaccine production and research of
hepatitis A virus.Methods:Use MILLIPORE PELLICON ultrafiltration device, PEG6000, sucrose - glycerin cushion three methods to
Hepatitis A liquid enrichment, Test concentrate antigen titer by ELISA, calculating the recovery rate of different methods.Results:Hepatitis A virus solution with ultrafiltration devices 7 times cycles, the average recovery was 86%. PEG concentration the average
recovery was 72.5%; sucrose-glycerol centrifuge enrichment method average recovery was 53.3% .Conclusion:Sucrose - glycerol
centrifuge enrichment method is purification concentration Synchronization and Suitable for small sample. PEG enrichment method is
Simple operation, and have wide range of applications. Ultrafiltration device can be effectively concentrated for large scale production,
but when the sample titer is high, it lead to sample loss. |
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