| 代海华 于海洋 陈丹丹 宋扬.刺参酸性粘多糖对H22 肝癌小鼠免疫功能的影响（英文）[J].,2014,14(23):4455-4458 |
| 刺参酸性粘多糖对H22 肝癌小鼠免疫功能的影响（英文） |
| Immune Effect of Stichopus Japonicus Acid Mucopolysaccharideon Hepatocarcinoma22-bearing Mouse |
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| DOI： |
| 中文关键词: 青岛大学医学院营养研究所 青岛市海慈医疗集团 |
| 英文关键词: Stichopus japonicus acid mucopolysaccharide Hepatocarcinoma22 Immune function |
| 基金项目:国家自然科学基金项目（810722951） |
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| 中文摘要: |
| 目的：刺参酸性粘多糖作为一种天然生物活性物质，具有较强抗肿瘤作用。本研究观察刺参酸性粘多糖对荷瘤小鼠免疫功
能的影响，以探讨刺参酸性粘多糖的抗肿瘤作用机制。方法：皮下接种H22 小鼠肝癌细胞，建立移植瘤小鼠模型。将50 只荷瘤小
鼠随机分为五组（阴性对照组、氟尿嘧啶组、SJAMP 低剂量组、SJAMP 中剂量组、SJAMP 高剂量组），腹腔注射不同剂量刺参酸性
粘多糖，每日一次，连续12 天。眼球摘除取血后颈椎脱臼处死小鼠，计算抑瘤率和脏器指数，中性红法测定小鼠腹腔巨噬细胞吞
噬功能，CCK-8 法测定小鼠脾淋巴细胞增殖能力，ELISA 法测定小鼠血清TNF-alpha水平。结果：SJAMP 能够明显抑制肿瘤生长
(P<0.05)；与5-FU组相比，SJAMP 干预组脾指数和胸腺指数明显升高(P<0.05)，腹腔巨噬细胞吞噬能力和脾脏淋巴细胞增殖功能
显著提高(P<0.05)，TNF-alpha的血清含量显著减少(P<0.05)。结论：刺参酸性粘多糖通过促进免疫器官生长，增强机体的免疫功能，抑
制小鼠H22 肝癌生长。这为SJAMP 的抗肿瘤作用研究提供了试验依据和理论基础。 |
| 英文摘要: |
| Objective:As a natural biologically active substance, Stichopus japonicus acid mucopolysaccharide (SJAMP) has
significant anti-tumor effect. The present study aimed to investigate the immune effect of SJAMP on Hepatocarcinoma22-bearing mouse.Methods:The mice were injected H22 cells into subcutaneous of the right armpit, then were randomly divided into model group, 5-FU
group and SJAMP intervention groups(group A, B, C). Three doses of SJAMP (6.25 mg/kg, 12.5 mg/kg, and 25 mg/kg administered once
a day via intraperitoneally inject ) were given to mice continuously for 12 days. Blood samples were drawn by excising eyeball and then
all the mice were sacrificed by cervical dislocation. The spleens and thymuses were removed under sterile conditions, and the spleen
indexes and thymus indexes were calculated. Neutral red method was used to detect macrophage phagocytosis. Spleen Lymphocytes
proliferation capability was assayed by CCK-8 method and TNF-alpha was detected by ELISA method.Results:SJAMP treatment
significantly inhibited Hepatocarcinoma22 by reducing the weight of tumor nodules. SJAMP administration also improved spleen indexes
and thymus indexes, abdominal macrophage phagocytosis and spleen Lymphocytes proliferation activity. Furthermore, SJAMP groups
showed a decrease in serumlevel of TNF-alpha.Conclusion:SJAMP effectively inhibits the growth of tumor through stimulating the immune
organs proliferation and enhancing immunity ability of Hepatocarcinoma22-bearing mouse. The results provide an experimental and
theoretical basis for the study of the anti-tumor effects of SJAMP. |
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