| 黄凤 段行武 董建勋 李健 赵明镜 荣培晶 徐旭英.经穴注射BMSCs联合中药对大鼠血清VEGF、G-CSF、SDF-1
浓度的影响[J].,2014,14(23):4409-4412 |
| 经穴注射BMSCs联合中药对大鼠血清VEGF、G-CSF、SDF-1
浓度的影响 |
| Effect of Concentration on VEGF, G-CSF, SDF-1 Stimulatedby Acupoint Injection BMSCs Combined Chinese Herbin Rat Serum |
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| DOI： |
| 中文关键词: 血管内皮细胞生长因子 粒细胞集落刺激因子 基质细胞衍生因子-1 |
| 英文关键词: VEGF G-CSF SDF-1 |
| 基金项目:北京市自然科学基金面上项目(7122084)；北京市卫生系统高层次卫生技术人才培养计划(2013-3-080)；
北京市科委首都临床特色应用研究(Z131107002213117)；北京市中医药科技年度规划项目(WZF2012-02) |
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| 中文摘要: |
| 目的：前期基础实验发现经穴注射骨髓间充质干细胞联合益气活血中药对大鼠缺血后肢骨骼肌血管密度以及后肢血流恢
复具有明显促进作用，为进一步明确其机制，本研究着重经穴注射骨髓间充质干细胞（BMSCs）联合益气活血方对大鼠血清中血
管内皮细胞生长因子（VGEF），粒细胞集落刺激因子（G-CSF），基质细胞衍生因子-1（SDF-1）浓度的影响，从而为临床中医血管外
科防治后肢动脉缺血性疾病提供新的思路和方法。方法：25只大鼠随机分为空白对照组（CG）、下肢缺血模型组（IG）、益气活血方
缺血组（HG）、经穴注射BMSCs 缺血组（BG），经穴注射BMSCs+ 益气活血方缺血组（BHG），每组5 只，在给予相应干预措施后，
于7 天后取血清，用酶联免疫吸附法（ELISA）测定大鼠血清中VEGF、G-CSF、SDF-1 质量浓度。结果：①与CG比较，IG、HG、BG、
BHG 大鼠血清中VEGF、SDF-1、G-CSF浓度显著升高（P＜0.01）；②与IG 比较，HG、BG、BHG 组大鼠血清中VEGF，G-CSF浓度
显著升高（P＜0.01），BG、BHG 大鼠血清中SDF-1 浓度显著升高（P＜0.01）；③与HG 比较，BG、BHG 大鼠血清中VEGF，SDF-1，
G-CSF 浓度显著升高（P＜0.01）；④与BG比较，BHG大鼠血清中VEGF，SDF-1，G-CSF浓度显著升高（P＜0.01），以上差异均有统
计学意义。结论：经穴注射BMSCs联合益气活血中药可大幅提升下肢缺血模型大鼠血清中VEGF、G-CSF、SDF-1 质量浓度，为
临床中医血管外科防治后肢动脉缺血性疾病提供了新的思路和方法。 |
| 英文摘要: |
| Objective:It was found in our early basic experiment that acupoint injection of bone marrow mesenchymal stem cells
combined Qi-tonifying and Blood-activating Chinese herb have obvious effect of promoting the vascular density and restoring the blood
flow to skeletal muscle of SD rat with hind limbs ischemic. In order to clarify the further mechanism, this study was designed to
investigate the concentration of vascular endothelial cell growth factor (VGEF), granulocyte colony stimulating factor (G-CSF), stromal
cell derived factor 1 (SDF-1) stimulated by acupoint injection of bone marrow mesenchymal stem cells (BMSCs) transplantation
combined Chinese Herb in rat serum, so as to provide new methods for prevention and cure arterial ischemic disease in vascular surgery
of traditional Chinese medicine.Methods:25 rats were randomly divided into control group (CG), lower limb ischemia model group(IG),
Herb group(HG), Acupoint injection of BMSCs ischemia group(BG), Acupoint injection of BMSCs +Herb ischemia group(BHG), and
given 7 days corresponding intervention measures. Then we measured the concentration of VEGF, G-CSF, SDF-1in rat serumby Enzyme
Linked Immunosorbent Assay (ELISA).Results:① Compared with CG, concentration of VEGF, G-CSF, and SDF-1 increased
significantly (P < 0.01) in IG, HG, BG, BHG; ②Compared with IG, concentration of VEGF and G-CSF increased significantly (P < 0.01)
in HG, BG, BHG, while SDF - 1 increased (P < 0.01) in BG, BHG; ③Compared with HG, concentration of VEGF, G-CSF, and SDF-1
increased significantly (P < 0.01) in BG, BHG; ④ Compared with BG, concentration of VEGF, G-CSF, and SDF-1 increased
significantly (P < 0.01) in BHG. The above all differences were statistically significant.Conclusion:Acupoint injection of BMSCs
combined Qi-tonifying and Blood-activating Chinese herb can be sharply raised the concentration of VEGF, G-CSF and SDF-1 in the
lower limb ischemia rat model, which provide new methods for prevention and cure arterial ischemic disease in vascular surgery oftraditional Chinese medicine. |
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