| 卢丽娜 吴红兵 苏靖 杨国汉 邹海淼 张君睿 邱明丰.蛙凝素修饰聚谷氨酸纳米粒的制备、表征及Calu-3 细胞摄取研究[J].,2014,14(22):4219-4222 |
| 蛙凝素修饰聚谷氨酸纳米粒的制备、表征及Calu-3 细胞摄取研究 |
| Preparation, Characterization and Calu-3 Cellular Uptake Research onOdorranalectin-conjugated PBLG Nanoparticles |
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| DOI： |
| 中文关键词: 蛙凝素 聚谷氨酸 纳米粒 细胞毒性 细胞摄取 |
| 英文关键词: Odorranalectin PBLG Nanoparticle Cytotoxicity Cellular uptake |
| 基金项目:国家自然科学青年基金项目（81001407） |
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| 中文摘要: |
| 目的：考察蛙凝素（Odorranalectin，OL）修饰对聚谷氨酸苄酯- 聚乙二醇纳米粒(PBLG-PEG-NPs)材料的Calu-3 细胞(人肺腺
癌细胞)毒性和细胞摄取作用的影响。方法：碘氧化法制备蛙凝素修饰聚合物材料；以姜黄素(curcumin，Cur)为模型药物，采用乳
化溶媒蒸发法制备聚谷氨酸苄酯- 聚乙二醇纳米粒(PBLG-PEG-NPs) 和蛙凝素修饰聚谷氨酸苄酯- 聚乙二醇纳米粒
(OL-PBLG-PEG-NPs)；MTT 法考察三种纳米粒对Calu-3 的细胞毒性；采用激光共聚焦显微镜对两种纳米粒的Calu-3 细胞摄取作
用进行定性观察。结果：给予高浓度(2 mg·mL-1)纳米粒培养Calu-3 细胞时，细胞存活率大于75 %。蛙凝素修饰纳米粒后其被细胞
摄取的量从62.7 %增加到了81.2 %。结论：OL可用于黏膜给药载体的修饰，OL-PBLG-PEG-NPs 细胞毒性低、生物相容性好，有望
成为一种鼻腔粘膜给药优良载体。 |
| 英文摘要: |
| Objective:To investigate the cytotoxicity and cellular uptake efficiency of OL modified PBLG-PEG-NPs
(PL-PBLG-PEG-NPs) compared with the non-modified one.Methods:OL-PEG-PLGA was synthesized using iodine oxidation method.
MTT assay was used to evaluate the cytotoxicity of Poly (r-benzyl-L-glutamate) block-ploy (ethylene glycol) nanoparticles
(PBLG-PEG-NPs) to Calu-3 cells. The cellular uptake of nanoparticles was visualized by confocal laserscanning microscope.Results:The cytotoxicity results indicated that cell viability had reached 75 % while Calu-3 cells were incubated at high concentration of 2 mg·
mL-1. After PBLG nanoparticles were odorranalectin-conjugated, the cellular uptake ratio raised from 62.7 % to 81.2 %.Conclusion:OL-PBLG-PEG-NPs had low cell cytotoxicity and good biocompatibility. It might be used for nasal mucosa drug delivery. |
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