| 贾谊君 林清 王传英 陆云姝 丁龙龙 程先华 吴克瑾.叶酸修饰稀土改性载氧碳纳米管增加乳腺癌细胞株放疗敏感性的
体外实验初步研究[J].,2014,14(22):4205-4209 |
| 叶酸修饰稀土改性载氧碳纳米管增加乳腺癌细胞株放疗敏感性的
体外实验初步研究 |
| Preliminary Study of Oxygen Carried Rare Earth Modified Folate-decoratedChitosan-SWCNTs Increase the Radiosensitivity of Human Breast CancerCell Lines In Vitro |
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| DOI： |
| 中文关键词: 乳腺癌 碳纳米管 乏氧 放疗 |
| 英文关键词: Breast cancer Carbon nanotubes Hypoxia Radiotherapy sensitivity |
| 基金项目:国家自然科学基金青年基金项目（81102016） |
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| 中文摘要: |
| 目的：研究叶酸修饰稀土改性载氧碳纳米管在低氧环境下对乳腺癌细胞株放疗敏感性的影响。方法：使用水溶性四唑盐法
(WST-1)方法研究叶酸修饰稀土改性载氧碳纳米管对MDA-MB-231 细胞与ZR-75-1 细胞生长的作用，使用细胞集落形成实验研
究其在低氧环境下对无叶酸培养基中MDA-MB-231 细胞、有叶酸培养基中MDA-MB-231细胞及ZR-75-1 细胞放疗敏感性的影
响。利用流式细胞术研究叶酸修饰稀土改性载氧碳纳米管联合放疗干预MDA-MB-231 乳腺癌细胞株的凋亡率的改变。利用
Western Blot实验观察Bcl-2、survivin、Hif-1alpha、Rad51 及Ku80 表达水平的改变。结果：在常氧及低氧环境下，叶酸修饰稀土改性载
氧碳纳米管在低于100 ug/ml的浓度时对乳腺癌细胞株生长无明显影响。在低氧环境下，放疗联合叶酸修饰稀土改性载氧碳纳米
管组相比于单纯放疗组细胞克隆形成率有不同程度的降低，以无叶酸培养基中MDA-MB-231 细胞组降低最为明显，照射剂量在
2、4、6、8Gy 时其细胞克隆形成率均显著降低（P<0.05）。流式细胞术显示叶酸修饰稀土改性载氧碳纳米管联合放疗后可使
MDA-MB-231 乳腺癌细胞株的凋亡率增加。Western Blot实验显示Bcl-2、Survivin、Hif-1alpha、Rad51 及Ku80 表达水平均降低。结论：
叶酸修饰稀土改性载氧碳纳米管可在体外低氧环境下增强乳腺癌细胞株对放疗的敏感性。 |
| 英文摘要: |
| Objective:To determine whether oxygen carried rare earth modified folate-decorated chitosan-SWCNTs (R-O2-FACHI-
SWCNTs) could increase the radiosensitivity of human breast cancer cell lines.Methods:WST-1 assay was used to determine the
cytotoxicity of the new synthesized R-O2-FA-CHI-SWCNTs on MDA-MB-231 and ZR-75-1 cells. MDA-MB-231 cells in folic acid free
medium, MDA-MB-231 cells in medium containing folic acid, and ZR-75-1 cells were treated with different doses of radiotherapy with
or without R-O2-FA-CHI-SWCNTs under hypoxic condition. Colony forming assay was used to determine the cell surviving fractions.
Cell apoptosis induction was monitored by flow cytometry. Expression of Bcl-2, Survivin, Hif-1alpha, Rad51 and Ku80 was monitored by
Western blotting.Results:The WST-1 assay revealed that R-O2-FA-CHI-SWCNTs had no significant cytotoxicity on breast cancer cells
when the concentration of R-O2-FA-CHI-SWCNTs was below 100 ug/ml under normoxic or hypoxic condition. Under hypoxic
condition, the administration of R-O2-FA-CHI-SWCNTs plus radiotherapy yielded lower colony forming efficiency compared to
radiotherapy alone, especially in MDA-MB-231 cells in folic acid free medium. The colony forming efficiency was reduced significantly
when the radiation dose was 2,4,6 and 8Gy. Flow cytometric analysis revealed that the R-O2-FA-CHI-SWCNTs plus radiotherapy could
increase the cell apoptosis rate of MDA-MB-231 cells. Furthermore, western blot demonstrated that the material exhibited its expected
function by downregulating Bcl-2, Survivin, Hif-1alpha, Rad-51 and ku80.Conclusion:The new synthesized R-O2-FA-CHI-SWCNTs could
significantly increase the radiosensitivity of human breast cancer cell lines in vitro. |
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