| 蔡春琳 周力学 曾亮 蒋芳清 陈跃飞.胆囊癌组织中IL-8 表达及TAM计数的临床病理意义[J].,2014,14(21):4071-4074 |
| 胆囊癌组织中IL-8 表达及TAM计数的临床病理意义 |
| Clinicopathological Significance of IL-8 Expression and TAMCount inGallbladder Carcinoma |
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| DOI： |
| 中文关键词: 胆囊癌 白细胞介素-8 肿瘤相关巨噬细胞 慢性胆囊炎 |
| 英文关键词: Gallbladder carcinoma Interleukin-8 Tumor associated macrophages Chronic cholecystitis |
| 基金项目: |
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| 中文摘要: |
| 目的：检测胆囊腺癌组织中白细胞介素-8(IL-8)mRNA的表达、肿瘤相关巨噬细胞(TAM)计数并探讨其临床病理意义。方法：
收集中南大学湘雅二医院及湖南省人民医院近五年胆囊腺癌手术切除标本36 例及慢性胆囊炎手术切除标本10 例，采用原位分
子杂交方法检测IL-8 表达，ABC免疫组化法进行TAM 计数。比较胆囊腺癌和慢性胆囊炎标本组织中IL-8 mRNA 表达和TAM
计数的差异，并分析其与胆囊腺癌临床病理特征之间的关系。结果：胆囊腺癌组织中IL-8 mRNA表达阳性率及其评分均明显高于
慢性胆囊炎(P<0.01)，IL-8 mRNA表达阳性率及其评分与其侵犯胆总管及发生淋巴结转移显著相关(P<0.05)。胆囊腺癌组织中
TAM 计数(24.89± 0.84)明显高于慢性胆囊炎组织(16.19± 0.66)，差异有统计学意义(P<0.01)；侵犯胆总管、肝脏及发生淋巴结转移
的胆囊腺癌组织中TAM 计数高于未侵犯胆总管、肝脏及发生淋巴结转移的胆囊腺癌组织TAM计数，其中侵犯胆总管和发生淋
巴结转移之间差异显著(P<0.01)。IL-8 mRNA阳性病例的TAM 计数均明显高于阴性病例(P<0.01)，TAM计数与IL-8 mRNA评分
间也存在显著正相关(r=0.748，P<0.001)。结论：IL-8 和TAM计数与胆囊癌的发生和发展密切相关，IL-8 可能在促进TAM向胆囊
癌组织迁移浸润中起作用。 |
| 英文摘要: |
| Objective:To investigate the expression of IL-8 mRNA and Tumor associated macrophages(TAM)count in gallbladder
carcinoma tissues and their clinical significance.Methods:36 cases of gallbladder carcinoma operation samples and 10 cases of Chronic
cholecystitis operation samples were collected from Hunan province Ren min Hospital and Second Xiangya Hospital. The expression of
IL-8 was detected by in situ hybridization method, TAMcount was determined by ABC immunohistochemical method. The difference of
IL-8 mRNA expression and TAM count were compared between gallbladder carcinoma and Chronic cholecystitis, and their relationship
with clinicopathological features of gallbladder carcinoma were analyzed.Results:The positive rate and score of IL-8mRNA expression
in gallbladder carcinoma tissues were significantly higher than those of the chronic cholecystitis tissues (P<0.01). The positive rate and
score of IL-8mRNA expression in gallbladder carcinoma tissues were significantly related to the infringement of common bile duct and
the incidence of lymph node metastasis. TAMcount of Gallbladder adenocarcinoma (24.89± 0.84) was significantly higher than that of
the chronic cholecystitis tissues(16.19± 0.66)(P<0.01). gallbladder adenocarcinoma TAMcount with violations of common bile duct, the
liver and lymph node metastasis were higher than those without violations of common bile duct, liver and lymph node metastasis with
significant differences (P<0.01). TAM count of IL-8 mRNA positive cases were significantly higher than those in IL-8 mRNA negative
cases (P<0.01).Conclusion:IL-8 and TAMcount were closely related to the occurrence and development of gallbladder carcinoma, and
IL-8 may promote TAMinfiltrating to gallbladder carcinoma tissue. |
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