| 王龙琦 宋林菲 郑恒睿 李荣贵.一株降解O,O-二甲基-O-(2,2- 二氯乙烯基)磷酸酯的土壤微生物的
分离与鉴定[J].,2014,14(21):4051-4055 |
| 一株降解O,O-二甲基-O-(2,2- 二氯乙烯基)磷酸酯的土壤微生物的
分离与鉴定 |
| Isolation and Identification of a DDVP-degrading Bacterial Strain fromSoil |
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| DOI： |
| 中文关键词: 细菌 O,O-二甲基-O-(2,2-二氯乙烯基)磷酸酯 降解 鉴定 |
| 英文关键词: Bacterium DDVP Degradation Identification |
| 基金项目:山东省自然科学基金项目（ZR2010CM009） |
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| 中文摘要: |
| 目的：有机磷农药和杀虫剂广泛应用于众多国内和国外生产的，其数量已超过100 种。大量使用的有机磷农药会增加农业
生产，而且还造成了不可估量的环境污染。研究降解敌敌畏的微生物，为微生物以降低产品敌敌畏农药残留，恢复敌敌畏污染土
壤中的研究奠定基础。方法：本文从种植蔬菜的温室大棚的土壤中分离了一株降解O,O- 二甲基-O-(2,2- 二氯乙烯基)磷酸酯(敌敌
畏)的细菌，根据该菌的形态学、生理生化特征及16S rDNA 序列比对。结果：该菌鉴定为荧光假单胞菌（菌株P）。该菌的最适生长
温度为27 ℃，其培养基的最适初始pH为7.0, 4天内该菌可将培养液中61.24%的降解。结论：本实验从蔬菜大棚的土样中筛选出
一株能降解敌敌畏的菌株，并鉴定为荧光假单胞菌。本研究将为基于微生物以降低产品敌敌畏农药残留，恢复敌敌畏污染土壤中
的研究打下基础。 |
| 英文摘要: |
| Objective:Organophosphorus pesticides and pesticide widely used in a large number of domestic and foreign production,
its number has more than 100 kinds. Heavily used organophosphorus pesticides can increase agricultural production, but also caused
incalculable environmental pollution. To research a microorganisms which can degrade DDVP for exploring microbial agents which can
decrease the residues of DDVP and restore the pesticides-polluted soils.Methods:A bacterium strain which could degrade one kind of
organophosphate pesticides, DDVP (dimethyl-dichloro-vinyl-phosphate), was isolated from soil samples collected from greenhouses
planting vegetables in Zibo, Shandong Province. The bacterium strain was identified as Pseudomonas flurescens strain P according to its
morphological, physiological, biochemical characteristics and 16S rDNA sequence blast.Results:The optimum growth temperature for
this strain was 27 ℃ , and the optimum initial pH was 7.0. P. flurescens strain P could degrade DDVP with an efficiency of 61.24% in
four days.Conclusion:This experiment screened a strain capable of efficient degradation of dichlorvos strains of Pseudomonas fluorescence
through the identification. This study will lay a foundation for exploring microbial agents which can decrease the residues of
DDVP and restore the pesticides-polluted soil. |
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