| 蔡春琳 周力学 曾亮 蒋芳清 陈跃飞.趋化因子MCP-1、MIP-1alpha的表达与TAM 计数在胆囊癌中的
临床病理意义[J].,2014,14(19):3706-3710 |
| 趋化因子MCP-1、MIP-1alpha的表达与TAM 计数在胆囊癌中的
临床病理意义 |
| Clinicopathological Significance of Expression of Chemokine MCP-1,MIP-1alpha and TAMCount in Gallbladder Carcinoma |
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| DOI： |
| 中文关键词: 胆囊癌 核细胞化学趋化蛋白-1 单核细胞炎性蛋白-1 肿瘤相关巨噬细胞 |
| 英文关键词: Gallbladder carcinoma MCP-1 MIP-1alpha Tumor associated macrophage |
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| 中文摘要: |
| 目的：检测胆囊腺癌组织中趋化因子MCP-1 和MIP-1-alpha的表达、TAM 计数并探讨其临床病理意义。方法：收集中南大学湘
雅二医院及湖南省人民医院近五年胆囊腺癌手术切除标本36 例及慢性胆囊炎手术切除标本10 例，采用原位分子杂交方法检测
MCP-1 和MIP-1alpha的表达，免疫组化法进行TAM计数。结果：胆囊腺癌组织中MCP-1、MIP-1alpha mRNA表达阳性率及评分均明显
高于慢性胆囊炎(P<0.01)；高分化胆囊腺癌中二者的阳性率及评分均低于低分化胆囊腺癌，其中MCP-1 mRNA 比较有显著性差异
(P＜0.05)；MCP-1、MIP-1-alpha-mRNA的表达呈显著正相关。胆囊腺癌组织MCP-1 mRNA表达阳性率及其评分与侵犯胆总管及发生
淋巴结转移显著相关；MIP-1alpha-mRNA表达阳性率及其评分与侵犯肝脏显著相关。胆囊腺癌组织中，TAM 计数(24.89± 0.84)明显
高于慢性胆囊炎组织(16.19± 0.66)，有显著性差异(P<0.01)。TAM与MCP-1、MIP-1alpha mRNA 表达评分值均呈显著正相关(r 分别为
0.580，0.567)。MCP-1mRNA 与MIP-1alpha-mRNA评分值之间呈显著正相关(r＝0.638)。结论：MCP-1、MIP-1alpha的表达增加及TAM 计
数升高可能调控和影响胆囊癌的发生和发展，MCP-1、MIP-1琢可能促进TAM向胆囊癌组织迁移浸润。 |
| 英文摘要: |
| Objective:Expression of MCP-1, MIP-1alpha and TAMcount in gallbladder carcinoma tissue were detected in this study,
and their clinical significance were analyzed.Methods:36 cases of gallbladder carcinoma operation samples and 10 cases of chronic
cholecystitis operation samples were collected fromHunan province People's Hospital and Second Xiangya Hospital. In situ hybridization
method was used to detect the expression of MCP-1and MIP-1alpha, ABC immunohistochemical method was used for TAM count.Results:In gallbladder carcinoma, the positive rate and score of MCP-1 and MIP-1琢mRNA expression was significantly higher than that of the
chronic cholecystitis(P<0.01); The positive rate and score of high differentiation gallbladder adenocarcinoma were lower than those of the
low differentiation gallbladder adenocarcinoma, while the MCP-1mRNA expressed significantly differently between different
differentiation groups (P<0.05); significant positive correlation was found betwween MCP-1 and MIP-1 alpha mRNA expression. In
gallbladder adenocarcinoma tissue, the positive rate and score of MCP-1 mRNA expression were significantly related to the infringement
of common bile duct and the incidence of lymph node metastasis; the positive rate and score of MIP-1 alpha mRNA expression were
significantly related to the invasion of the liver. In 36 cases of gallbladder carcinoma tissue, TAMcount (24.89± 0.84) was significantly
higher than that of 10 cases of chronic cholecystitis group (16.19± 0.66) with significant difference (P<0.01). TAMcount of MCP-1 and
MIP-1alpha mRNA positive cases were significantly higher than those in negative cases (P<0.01). TAM and MCP-1, MIP-1 alpha mRNA
expression score showed significant positive correlation(r=0.580, 0.567). Score of MCP-1mRNA and MIP-1alpha mRNA has a close positive
correlation (r=0.638).Conclusion:The increased expression of MCP-1, MIP-1 alpha and TAM count enhencement may regulate and
influence the occurrence and development of gallbladder carcinoma, MCP-1, MIP-1alpha may promote TAMto gallbladder carcinoma tissue
infiltration. |
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