| 习攀 朱美玲 顾文华 王一玮 薛文姬 郑磊贞.热疗对SW1990 胰腺癌细胞上皮间质转化（EMT）的影响及其作用机制[J].,2014,14(19):3611-3614 |
| 热疗对SW1990 胰腺癌细胞上皮间质转化（EMT）的影响及其作用机制 |
| Influence and Mechanisms of Hyperthermia on the Epithelial MesenchymalTransformation(EMT) of SW1990 CellsInfluence and Mechanisms of Hyperthermia on the Epithelial MesenchymalTransformation(EMT) of SW1990 Cells |
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| DOI： |
| 中文关键词: 吉西他滨 SW1990 上皮间质转化 E-钙粘素 Notch1 |
| 英文关键词: Hyperthermia SW1990 EMT E-cadherin Notch1 |
| 基金项目:上海市科委产学研医合作项目（11DZ1922007） |
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| 中文摘要: |
| 目的：探讨热疗对化疗诱导的人胰腺癌细胞株SW1990 细胞上皮间质转化（EMT）的影响及其可能的作用机制。方法：分别
用不同浓度吉西他滨(0, 5, 10, 20，30 滋M)作用于SW1990 细胞不同时间(24, 48, 72 小时)及30 滋M 吉西他滨作用24h 联合热疗
43℃ 1h，观察细胞的形态学变化，通过四甲基偶氮唑蓝(MTT) 法检测细胞的增殖情况。采用Western blot 方法检测细胞内
E-cadherin 蛋白和剪切的Notch1 蛋白的表达。结果：①吉西他滨在一定浓度范围内可明显抑制SW1990细胞的增殖（P<0.05），并
呈浓度和时间依赖性。吉西他滨作用前24 h 给予43℃ 1h热疗预处理可显著增强吉西他滨对SW1990细胞的抑制作用（P<0.05）
②吉西他滨作用SW1990 细胞24 小时后，细胞数目减少，细胞形态变大，细胞呈梭形，且细胞间连接减少；而热疗预处理的联合
能够逆转此种形态学变化。③吉西他滨作用24 小时后，细胞内E-cadherin蛋白的表达下调、Cleaved Notch1 的蛋白表达上调，热
疗预处理可明显上调吉西他滨诱导的E-cadherin 蛋白表达下调、并下调Cleaved Notch1 蛋白表达的上调。结论：热疗预处理显著
逆转吉西他滨所诱导的人胰腺癌细胞株SW1990 细胞的EMT 现象，其机制可能与Notch 信号通路有关。 |
| 英文摘要: |
| Objective:To investigate the influence of hyperthermia on epithelial mesenchymal transformation (EMT) induced by
chemotherapy in pancreatic cancer cells and its mechanism.Methods:SW1990 cells were treated with Gemcitabine in various
concentrations (0, 5, 10, 20,30 uM) for different durations (24, 48 and 72 h) or 30 uMgemcitabine combined with hyperthermia 43 ℃ 1
h．The cell morphological changes were observed, the proliferation of SW1990 cell was detected by MTT method, the expression of
E-cadherin protein and Cleaved Notch1 protein in cell were assayed by Western blot.Results:①SW1990 cell proliferation was inhibited
in certain concentrations with a time and dose-dependent manner after treatment of gemcitabine (P<0.05); Hyperthermia 43 ℃ 1h
pretreatment 24h before treatment of gemcitabine on SW1990 cell can enhance the gemcitabine inhibited proliferation of SW1990 cells
(P<0.05) ②When treated with 30 u Mgemcitabine, the cells became larger，showed a spindle shape and lost cell adhere. However, which
were partially reversed by pretreatment with hyperthermia. ③The protein expression of E-cadherin reduced and Cleaved Notch1 protein
up-regulated in SW1990 cells treated with gemcitabine for 24 hours; hyperthermia pretreatment can up-regulate the expression of
E-cadherin protein and reduce the up-regulation of Cleaved Notch1 protein induced by gemcitabine.Conclusion:Hyperthermia
pretreatment could obviously reverse the gemcitabine induced EMT in SW1990 cell, which may be related to the Notch pathway. |
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