| 杨帆1 李冰1△ 徐颖婕1 吕丛仪1 褚现明2 聂淑敏3.藻蓝蛋白联合全反式维甲酸对HeLa 细胞生长抑制作用的研究*[J].,2014,14(17):3248-3254 |
| 藻蓝蛋白联合全反式维甲酸对HeLa 细胞生长抑制作用的研究* |
| Inhibitory Effects of C-phycocyanin Combined with All-transretinoicAcid on the Growth of HeLa Cells* |
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| DOI： |
| 中文关键词: 全反式维甲酸 藻蓝蛋白 HeLa细胞 凋亡机制 |
| 英文关键词: All-transretinoic acid C-phycocyanin HeLa cells Apoptosis mechanism |
| 基金项目:山东省医药卫生发展项目（2011HZ023） |
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| 中文摘要: |
| 摘要目的：探讨全反式维甲酸和藻蓝蛋白单独及联合用药对HeLa 细胞生长的影响，并揭示两者联合用药对细胞周期和细胞凋
亡影响的分子机制。方法：MTT 法检测全反式维甲酸和藻蓝蛋白单独及联合用药对HeLa 细胞生长的影响，原位杂交法检测用药
前后细胞内CDK-4基因mRNA 的表达情况，免疫组化法检测用药前后bcl-2 基因的表达情况，TUNEL法检测用药前后细胞凋亡
情况。结果：全反式维甲酸和藻蓝蛋白均具有抑制HeLa 细胞生长的作用，当达到相同的抑制率时，联合藻蓝蛋白使用可以显著
降低全反式维甲酸的使用剂量从而达到降低毒副作用的目的。两者联合用药可以显著降低CDK-4的表达量从而对HeLa 细胞的
细胞周期产生影响。两者联合用药可以显著下调bcl-2 的表达水平从而引发细胞凋亡。结论：通过联合藻蓝蛋白，可以显著降低全
反式维甲酸的使用剂量从而降低毒副作用。全反式维甲酸和藻蓝蛋白联合用药抑制HeLa 细胞生长的分子机制可能是通过抑制
CDK-4 和bcl-2 的表达来影响细胞周期并最终导致细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective:We focused on studying the effects of All-transretinoic acid (ATRA), C-phycocyanin (C-PC) or ATRA +
C-PC on HeLa cell growth, cell cycle distribution and apoptosis.Methods: MTT assay was adopted to determine the effects of C-PC
and/or ATRA on the growth of HeLa cells. The expressions of CDK-4 and Bcl-2 were determined by in situ hybridization and
immunohistochemistry staining. TUNEL assay was used to analyze the cell apoptosis. Results:Both C-PC and ATRA could inhibit the
growth of HeLa cells. And these two drugs showed synergistically inhibitory effects. The dosage of ATRA was reduced when used with
C-PC. ATRA, combined with C-PC, was more efficient at inducing cell cycle arrest by decreasing CDK-4 expression. The combination
of the two drugs could lead to down-regulation of Bcl-2 expression and thereby cell apoptosis.Conclusion: By combined with C-PC,
ATRA can be used at lower dosage with reduced toxicity. C-PC + ATRA combination might exert the inhibitory effects on cell growth
by inducing cell cycle arrest and cell apoptosis. |
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