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目的：观察异丙酚对人肺动脉内皮细胞中ACE2 的影响。方法：以人肺动脉内皮细胞为研究对象，利用Real-time PCR 检测不同浓度（1、10、20、40、50 umol/L）异丙酚在不同时间点（6、12、18、24、30h）对HPAEC 中ACE2 mRNA 表达的影响；Western Blot 检测不同浓度（1、10、20、40、50 umol/L）异丙酚对HPAEC 中ACE2 蛋白表达的影响；观察磷脂酰肌醇-3- 激酶（PI3K）抑制剂 LY294002 对异丙酚调节HPAEC 中ACE2 mRNA 表达的影响。结果：异丙酚呈剂量和时间依赖性可提高人肺动脉内皮细胞 ACE2mRNA 水平(P<0.05)。但异丙酚浓度为1 umol/L时对ACE2 mRNA 水平表达无明显影响(P>0.05)。Western Blot检测结果显示异丙酚可增加HPAEC中ACE2 蛋白的表达，且在24 h内具有剂量依赖性。异丙酚在24h 时剂量依赖性提高Akt 的磷酸化，而 LY294002 可逆转异丙酚对Akt磷酸化的影响。结论：异丙酚可分别通过PI3K/Akt 信号途径上调ACE2 的表达，使RAS 轴处在动态平衡中，从而发挥舒张血管和镇痛作用。

英文摘要:

Objective: To observe the effect of propofol on Angiotensin-Converting Enzyme 2 (ACE2) expression of human pulmonary artery endothelial cells.Methods:Original generation human pulmonary artery endothelial cells (HPAEC) were taken as the objects. Real-time PCR were used to detect the effect of different concentrations (1, 10, 20, 40,50 umol/L) of propofol for different time (6,12,18,24,30h) on theACE2 mRNA expression in HPAEC; Western blot was also applied to detect the ACE2 protein expression in HPAEC after treated by propofol of different concentrations (1,10,20,40,50 mmol/L). Observe the effect of phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 on the regulation of propofol.Results:Propofol increased the ACE2 mRNA levels of treated cells in doseand time-dependent manner (P<0.05), but had no effect on the ACE2 mRNA level when with a concentration of 1 umol/L. Western Blot detection results showed that propofol could increase the HPAEC ACE2 protein expression, and in a dose-dependent manner within 24 hours. In 24 hours, propofol increased the Akt phosphorylation dose-dependently, and LY294002 down-regulated the phosphorylation of Akt.Conclusion:Propofol increased the expression of ACE2 through the PI3K/Akt signaling pathways and rebalanced RAS axis, and thus played a role in relaxing blood vessels and had analgesic effects.

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