| 朱晓斐 李江 王宝 李玉骞 赵振伟.阿魏酸钠对人动脉平滑肌细胞和内皮细胞作用的机制研究[J].,2014,14(16):3046-3050 |
| 阿魏酸钠对人动脉平滑肌细胞和内皮细胞作用的机制研究 |
| Effects and Mechanisms of SodiumFerulate on Human Aortic SoomthMuscle Cells and Endothelial Cells |
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| DOI： |
| 中文关键词: 阿魏酸钠 血管细胞 抑制再狭窄 |
| 英文关键词: HASMCs HAECs In-stent restenosis |
| 基金项目:国家自然科学基金项目(81101022) |
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| 中文摘要: |
| 目的：研究阿魏酸钠（SF）对人主动脉平滑肌（HASMC）和内皮细胞（HAEC）的影响，探讨SF 成为抑制支架内再狭窄药物的
机制。方法：HASMC 和HAEC经SF 处理后（0-1000 ug/ml），用CCK-8 试剂和划痕愈合试验检测不同药物浓度对两种生长和细
胞迁移能力的影响；采用免疫细胞化学和Western blot检测HAECs中FoxM1 和VEGF的表达。结果：SF对两种细胞的作用呈剂
量依赖性，SF在10-1000 ug/ml 浓度时，HASMC 的生长活力明显降低，在0.1-100 ug/mlHAECs 生长活力显著增强（P＜0.05）。在
1-1000 ug/ml 浓度下HASMCs 迁移能力受到抑制，HAECs 的迁移能力明显增加（0.1-100 ug/ml）（P ＜ 0.05）。同时HAECs 内
FoxM1 和VEGF表达明显增高（P＜0.05），程度与SF浓度有剂量依赖关系。结论：阿魏酸钠能抑制血管平滑肌的增值和迁移；同
时增加内皮细胞FoxM1 和VEGF的表达，促进内皮细胞的增值和迁移，这些特征使其可能成为抑制支架内再狭窄的药物。 |
| 英文摘要: |
| Objective: To study the effects and mechanisms of Sodium ferulate (SF) on human aortic soomth muscle cells
(HASMCs) and endothelial cells (HAECs) to approach its potential possibility for in-stent restenosis.Methods:HASMCs and HAECs
were treated with different concentrations of SF and divided into 6 groups (0 ug/ml, 0.1 ug/ml, 1 ug/ml, 10 ug/ml, 100 ug/ml, and 1000
ug/ml).Cell viability was determined by CCK-8 kit. Cell migration distance was evaluated by Scratch wound healing assay. Changes of
FoxM1 and VEGF expression in HAECs were detected through the Immunocytochemistry method, Western blot and image analysis.Results:SF inhibited proliferation and migration of HASMCs in a does-dependent manner (10-1000 ug/ml)（P＜0.05）. However, SF
promoted proliferation and migration of HAECs in a dose-dependent manner (0.1-100 ug/ml)（P＜0.05）. The expression of FoxM1 and
VEGF was increased in a does-dependent manner in HAECs （P＜0.05）.Conclusion:SF may inhibit proliferation of HASMCs, and
promote vascular endothelial healing through increasing the expression of FoxM1 and VEGF. It suggests that SF may be an effective
agent for in-stent restenosis. |
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