| 曹留霞张静刘晓萍△ 卞晶晶陈琛张代军.UBC9 在人肺癌组织中高表达及其临床意义*[J].,2014,14(15):2947-2951 |
| UBC9 在人肺癌组织中高表达及其临床意义* |
| Higher Expression of Ubc9 in Human Lung Cancer Tissues and Its clinicalSignificance* |
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| DOI： |
| 中文关键词: 肺癌 Ubc9 临床特征 |
| 英文关键词: Lung cancer Ubc9 Clinical characteristics |
| 基金项目:山东省自然科学基金项目资助（ZR2012CM008） |
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| 中文摘要: |
| 摘要目的：由于Ubc9 在肿瘤的发生于恶化中发挥巨大作用，本研究目的是对泛素结合酶（Ubc9）mRNA和Ubc9 蛋白在非小细
胞肺癌组织中表达进行检测，评估Ubc9在肺癌预后中的指导意义。方法：通过荧光定量PCR、免疫组织化学法、Western-blot检测
100例非小细胞肺癌病人中Ubc9 mRNA、蛋白水平的表达进一步研究Ubc9的表达与肺癌临床特征的关系。结果：实验结果显示
在肺癌细胞中Ubc9 阳性表达显示为黄棕色颗粒，与癌旁组织比较，Ubc9 mRNA、蛋白在肺癌组织中高表达，并且与肺癌的临床分
型（分期、淋巴结转移、吸烟、分化）有关。Ubc9 mRNA、蛋白在肺癌中的表达癌组织高于癌旁、有淋巴结转移的高于无转移、吸烟高
于不吸烟者、低分化组织高于高分化组织。结论：由此可见Ubc9 mRNA、蛋白水平的高表达可能对肺癌临床特征的评估，以及预
测术后生存率都有重要指导意义。 |
| 英文摘要: |
| ABSTRACT Objective:As Ubc9 may play a great role in tumor genesis and deterioration, the purpose of this study was to detect the
expression of ubiquitin-conjugating enzyme (Ubc9) and assess the prognostic significance of Ubc9 in lung cancer. Methods:The expression
of Ubc9 mRNA and Ubc9 protein in Non-small cell lung cancer and para-carcinoma tissue from 100 lung cancer patients were detected
using Real-time, Immunohistochemistry and Western-blot. The correlation of Ubc9 expression with the clinical characteristics was
analyzed by the single factor correlation analysis.Results: The Ubc9 protein stained brown granules in cytoplasm and nucleus of cancer
cells. The higher expression of Ubc9 mRNA and Ubc9 protein were detected in cancer tissue than that in para-carcinoma tissue and correlated
with TNMstage, lymph nodes metastases, differentiation and smoking.Conclusion: The expression level of Ubc9 mRNA or protein
may independently predict postsurgical survival in lung cancer. |
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