| 杨帆吴琼康杨李雪胡琦△.共聚焦显微镜对蒸发过强型干眼眼表结构的观察*[J].,2014,14(15):2893-2896 |
| 共聚焦显微镜对蒸发过强型干眼眼表结构的观察* |
| In Vivo Study of Evaporative Dry Eye Using Laser Confocal Microscope* |
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| DOI： |
| 中文关键词: 激光共焦显微镜 蒸发过强性干眼 眼表 |
| 英文关键词: Laser confocal microscope Evaporative dry Ocular surface |
| 基金项目:哈尔滨市科研创新人才研究专项资金项目（2011RFXYS051） |
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| 中文摘要: |
| 摘要目的：干眼已成为影响人们生活质量的主要眼表疾病之一。有关干眼的研究已成为当今眼科的研究热点，本文通过激光共
焦显微镜对蒸发过强型干眼患者眼表结构特征进行观察，从细胞层面对干眼进行研究。方法：海德堡激光共焦显微镜(HRT3)对
35 例(60 只眼)干眼患者及35 名(60 只眼)正常人的角膜上皮层朗格汉斯细胞，睑板腺腺泡密度，睑板腺开口直径及形态进行观
察，并对观察结果进行描述记录。结果：正常眼角膜中央上皮层朗格汉斯细胞(LC)个数的平均密度为58± 19 个/mm2,干眼组为
(137± 29)个/mm2，两者比较差异具有统计学意义(P<0.05)。正常眼组角膜上皮下朗格汉斯细胞突起较短，分支较少，干眼患者角
膜上皮下朗格汉斯细胞突起呈树支状，其突起较正常眼组数量多且长度较长，正常组睑板腺(MG)腺泡的平均密度为115± 28 个
/mm2，干眼患者的睑板腺腺泡的平均密度59± 16 个/mm2，两者比较差异具有统计学意义(P<0.05)。干眼组睑板腺腺泡内呈中等程
度反光，对照组腺泡内呈低反光。正常组睑板腺开口呈圆形，内壁光滑，直径为62± 14 μm，干眼组睑板腺开口欠光滑，直径35±
11 μm，两者比较差异具有统计学意义(P<0.05)。结论：共聚焦显微镜观察到在蒸发过强型干眼患者中，睑板腺腺泡直径增大，密度
降低，睑板腺的开口直径变小，同时，角膜朗格汉斯细胞活化，数量增多，共聚焦显微镜可从细胞层面观察蒸发过强性干眼的眼表
结构改变，使干眼病理变化的研究更为直观，为今后的进一步研究提供了更为准确的材料。 |
| 英文摘要: |
| ABSTRACT Objective:Dry eye symptoms is a disease, therefore timely detection may lead to recognition of a life-threatening condition.
This article will evaluate the changes of ocular surface with evaporative dry eye using laser confocal microscope. Methods:60
eyes of 35 patients who were diagnosed as evaporative dry eye, were chosen as the study group of dry eye. Other 60 eyes of 35 patients
control subjects were included in the study. The images of langerhans cell (LC), meibomian gland (MG) acinar unit density and the diameter
of MG orifices were acquired by laser confocal microscope.Results: The langerhans cell density was 137± 29cells/mm2 in dry eye
group, compared with control group 58± 19 cells/mm2 . There was statistical difference accordingly between the two group(P<0.05). The
processes of langerhans cell in dry eye were longer and showing more activity than that were in control group. The acinar unit density
was 59± 16 cells/mm2 in dry eye group, compared with control group 115± 28 cells/mm2. There was statistical difference accordingly
between the two group (P<0.05). The diameter of MG orifices was 35± 11 μm, compared with control group 62± 14 μm. There was statistical
difference accordingly between the two group (P<0.05).Conclusion: The density of langerhans cell increased in dry eye group,
The processes of langerhans cell in dry eye group were longer and showing more activity than that were in control group. The density of
acinar unit declined in dry eye group. Laser confocal microscopy is valuable for basic research and clinical diagnosis of evaporative dry
eye. |
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