| 林德伟1 吴炜霖2 曾嵘3△ 谢素嫣1 谢妍1 杨冬宇1.MDA-DHPLC技术在PGS中的应用*[J].,2014,14(15):2875-2878 |
| MDA-DHPLC技术在PGS中的应用* |
| Application Research ofMDA-DHPLCin Preimplantation Genetic Screening* |
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| DOI： |
| 中文关键词: 种植前遗传学筛查（PGS） 丙二醛（MDA） 变性高效液相色谱（DHPLC） |
| 英文关键词: Preimplantation genetic screening Malondialdehyde Denaturing High performance liquid chromatography |
| 基金项目:广东省科技攻关项目（2011B032000004） |
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| 中文摘要: |
| 摘要目的：评估丙二醛变性高效液相色谱法在胚胎移植过程中的临床价值。方法：收集我院生殖中心2010 年2 月～2012 年3 间
未做移植的废弃胚胎，由D2胚胎培养到D3 胚胎期，提取遗传物质并进行PRC扩增，采用高形态学评分的D3 作为对照，高效液
相色谱法测定遗传DNA过氧化产物丙二醛（MDA）的含量。结果：多核Ⅰ组D2 期卵裂球核≥ 2 个，共24枚胚胎，DNA过氧化产
物丙二醛（MDA）的含量5.32± 0.19 μmol/L，对比正常胚胎0.67± 0.08 μmol/l有显著差异（P＜0.05）。多核Ⅱ组D2 期卵裂球核
=2，共19 枚胚胎，MDA的含量4.12± 0.22 μmol/L，对比正常胚胎0.67± 0.08 μmol/l 有显著差异（P＜0.05）。Ⅰ组与Ⅱ组间没有统
计学差异。然而，空泡胚胎与非空泡胚胎之间MDA并没有差异。结论：多核D2 期胚胎培养到D3 期会产生很高异常率，因此临床
上应减少使用此种进行移植。 |
| 英文摘要: |
| ABSTRACT Objective:To assess the application of MDA-DHPLC in the process of Embryo transferation.Methods: Select the
abandoned D2 embryo from reproductive center of our hospital between the period of Feb.2010 to Jan.2012. Detect the content of the
Peroxidation products MDA on DNA by DHPLC, take the good morphology D3 group as control. Results:There were 24 embryos in
groupⅠwhose D2 embryo is Multinucleated and blastomere nucleus ≥ 2, Peroxidation products MDA on DNA detected by DHPLC was
5.32 士0.19 μmol/l, compared with the controlled group 0.67± 0.08 μmol/L(P＜0.05); 24 D2 embryos in groupⅡ were Multinucleated
and blastomere nucleus=2, Peroxidation products MDA was 4.12± 0.22 μmol/L, compared with the controlled group 5.32± 0.19滋mol/l
(P＜0.05). There were no statistical deference between groupⅠand groupⅡ(P>0.05). Neither between Multinucleated Vacuoles embryos
and non-Vacuoles embryos.Conclusion: Development of Multinucleated D2 embryos to D3 derives high abnormal rates, we avoid to use
those embryos to conduct Embryo transplantation. |
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