| 杨海1 钱静1 蒋小琴1 舒荣文2 王讯1.miR-188-5p 对肾间质纤维化过程中MMP-13表达的影响*[J].,2014,14(15):2848-2852 |
| miR-188-5p 对肾间质纤维化过程中MMP-13表达的影响* |
| Effect of miR-188-5p on the Expression of MMP-13 During RenalInterstitial Fibrosis in Mice* |
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| DOI： |
| 中文关键词: 肾间质纤维化 基质金属蛋白酶 miR-188-5p |
| 英文关键词: Renal interstitial fibrosis Matrixmetalloproteinases miR-188-5p |
| 基金项目:国家自然科学基金项目（31301127） |
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| 中文摘要: |
| 摘要目的：探讨miR-188-5p 对肾间质纤维化过程中基质金属蛋白酶(matrixmetalloproteinases，MMPs)表达的影响。方法：采用
TGF-β1 诱导肾小球系膜细胞(Mesangial cells，MC)纤维化，观察纤维化过程中miR-188-5p 和MMP-13 的表达变化；通过报告基因
实验研究miR-188-5p 对MMP-13 的调控作用；通过脂质体转染的方法将人工合成的miR-188-5p mimics/inhibitor 转入细胞内增
加或减少miR-188-5p 的表达，进一步观察miR-188-5p 对MMP-13 表达的影响。结果：TGF-β1 诱导肾小球系膜细胞纤维化后
miR-188-5p 的表达明显升高，而MMP-13表达下调；报告基因实验表明miR-188-5p 对MMP-13 的表达有明显的调控作用，减少
miR-188-5p 的表达后MMP-13 的表达上调，而增加miR-188-5p 的表达后MMP-13 的表达下调，呈明显负性调控。结论：在肾间质
纤维化过程中，miR-188-5p 可能通过抑制MMP-13 的表达而发挥促纤维化的作用，本研究为肾间质纤维化的治疗提供了新的靶
点。 |
| 英文摘要: |
| ABSTRACT Objective:To explore the role of miR-188-5p on the expression of matrixmetallo- proteinases 13 (MMPs-13) during
renal interstitial fibrosis in mice.Methods: Mice renal mesangial cells (MC) were treated with TGF-β1 at concentration of 2ng/ml for
24h, then the mRNA and protein level of miR-188-5p and MMP-13 were tested. Next, reporter gene experiment was used to verify the
effect of miR-188-5p on the regulation of MMP-13. In order to observe the effect on MMP-13, level of miR-188-5p in MC cell were then
up/down regulated by mimics/inhibitor through Lipofectamine transfection.Results: TGF-β1 increased the expression of the miR-188-5p,
while the mRNA and protein level of MMP-13 were reduced. Reporter gene experiment confirmed the negative regulation of miR-188-5p
on MMP-13 expression. Up-regulation of miR-188-5p could inhibit the expression of MMP-13, while down-regulation of miR-188-5p
could increade the level of MMP-13. Conclusion:In renal interstitial fibrosis, miR-188-5p may play a fibrogenic role by down-regulating
the expression of MMP-13. This study provided a new therapeutic target for renal interstitial fibrosis. |
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