| 吴凌云王中△ 陈罡季骋远王伟.褪黑素对蛛网膜下腔出血后继发性脑损伤及认知功能的影响及
其机制研究*[J].,2014,14(15):2823-2826 |
| 褪黑素对蛛网膜下腔出血后继发性脑损伤及认知功能的影响及
其机制研究* |
| Research on the Effects and Mechanisms of Melatonin on the Secondarybrain Damage and Neurobehavioral Dysfunction after ExperimentalSubarachnoid Hemorrhage* |
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| DOI： |
| 中文关键词: 蛛网膜下腔出血 褪黑素 脑损伤 Morris水迷宫试验 认知功能 |
| 英文关键词: Subarachnoid hemorrhage Melatonin Brain injury Morris water maze task Cognitive function |
| 基金项目:国家自然科学基金项目（81100872） |
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| 中文摘要: |
| 摘要目的：探讨褪黑素对蛛网膜下腔出血(subarachnoid hemorrhage，SAH)后神经元细胞凋亡、坏死及继发性认知功能障碍的影
响。方法：选择80 只成年健康雄性SD 大鼠，随机分为四组：正常组(n=20)、单纯SAH 组(n=20)、SAH+ 安慰剂治疗组(n=20)和
SAH+褪黑素治疗组(n=20)，经大鼠自体尾动脉(股动脉)非肝素化动脉血在20 s内注入视交叉池建立蛛网膜下腔出血模型，褪黑
素注射剂量为150 mg/kg，1次/12 h，在蛛网膜下腔出血建模后48h处死各组部分大鼠，取血凝块周围的皮层脑组织(额颞底)做标
本，通过TUNEL荧光染色及Fluoro-Jade B荧光染色测定神经元凋亡及坏死的情况，各组剩余大鼠在SAH后48 小时开始通过
Morris 水迷宫试验测试其认知功能。结果：SAH组大鼠的活动功能评分、Morris 水迷宫试验的逃避潜伏期及总路程、神经元细胞
凋亡和坏死的百分比均较正常对照组大鼠显著升高(P<0.01)，而褪黑素治疗组以上指标均显著低于安慰剂治疗组(P<0.05)，但
SAH组和安慰剂组之间以上指标比较均无统计学意义(P>0.05)。结论：褪黑素可能通过减少神经元细胞的凋亡和坏死改善蛛网膜
下腔出血后大鼠的认知功能障碍。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effects and mechanisms of melatonin on the secondary brain damage and neurobehavioral
dysfunction after experimental subarachnoid hemorrhage (SAH). Results:80 adult SD rats were divided into four groups: control
group (n=20), SAH group (n=20), SAH+vehicle group (n=20), and SAH+melatonin group (n=20). The rat SAH model was induced by
injection of 0.3 ml fresh arterial, nonheparinized blood into the prechiasmatic cistern in 20 s. In SAH+melatonin group, melatonin was
administered i.p. at 150 mg/kg at 0, 12, 24 and 36 hr after the induction of SAH. Part of rats in each group were killed on 48 hr after
SAH.The brain sample was removed and keeped in 10%formalin solution.Cognitive and memory changes were investigated in the Morris
water maze at 48 hr after the induction of SAH with the rest of rats in each group. Results:The behavior and activity scores, escape latency
and swimming distance in Morris water maze task, apoptosis and necrosis percentage of neurons of SAH rats were all significantly
higher than those of the contol rats (P<0.01), while the above index of SAH+melatonin group were significantly lower than those of the
SAH+vehicle rats (P<0.05), but no significant difference was found in the above index between SAH and SAH+vehicle rats(P>0.05).
Methods:Melatonin may improve the neurobehavioral dysfunction after SAH through decreasing the apoptosis and necrosis of neurons. |
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