| 车莉萍1 程超2 张桉瑜2 冯菲2 崔斌2 孙高峰2 张建2 左长京2△.循环血游离Shope病毒DNA含量与兔VX2 肿瘤18F-FDG PET/CT 影像
表现的相关性分析*[J].,2014,14(15):2808-2811 |
| 循环血游离Shope病毒DNA含量与兔VX2 肿瘤18F-FDG PET/CT 影像
表现的相关性分析* |
| The Correlation between Cell-Free Shope Virus DNA Circulating in Plasmaand 18F-FDG PET/CT Imaging in VX2 Tumor-Bearing Rabbits* |
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| DOI： |
| 中文关键词: VX2 肿瘤 循环血游离DNA 氟[18F]-脱氧葡萄糖 正电子发射计算机断层 标准摄取值 |
| 英文关键词: VX2 tumor ccfDNA (circulating cell free DNA) 18F-FDG PET（Positron Emission Tomography） SUV（Standard
Uptake Value） |
| 基金项目:：上海市卫计委“新百人计划”基金（XBR2011040）；上海市博士后科学基金(11R21410600)；上海市人才发展资金(2010020)；
长海医院“1255”学科建设基金（CH125521103） |
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| 中文摘要: |
| 摘要目的：探讨循环血中Shope 病毒DNA含量与兔VX2 肿瘤18F-FDG PET/CT 影像学特征间的关系及其临床意义。方法：采用
组织块接种法建立兔VX2肿瘤模型，并行18F-FDG PET-CT 观测肿瘤大小及糖代谢相关值，实时定量荧光探针PCR 法检测肿瘤
组织及血浆中Shope 病毒特征性DNA 片段含量。结果：移植前外周血中未检测出Shope 病毒特异性DNA片段；移植后2周，
VX2 肿瘤组织和循环血中均可以检测到特征性Shope 病毒DNA 片段。瘤体内DNA 含量明显高于循环血中含量。循环血Shope
病毒DNA 含量与FDG-PET 的最大标准摄取值（SUVmax）明显呈正相关（r=0.943，p=0.005），但与肿瘤体积相关性尚不明确（r=
0.657，p=0.156）。结论：循环血Shope 病毒DNA有望作为一种潜在的VX2 肿瘤标志物，其廉价、无创的特性，有望在肿瘤的早期
诊断和预后随访中发挥优势。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the relationship between 18F-FDG PET/CT imaging characteristics and circulating cell-free
shope virus DNA copies, and to explore its clinical values. Methods:The VX2 tumor models in rabbit were established by transplanting
VX2 tumor mass into leg muscles, and the shope virus DNA in tumor tissues and plasma was quantified by a quantitative real-time
quantitative polymerase chain reaction (PCR) method.Results: Before tumor transplantation, no viral DNA was detected in peripheral
blood; 14 days after transplantation circulating shope virus specific DNA fragments could be detected. Concentration of shope virus DNA
in tumor tissue (mean (4.9± 1.9)× 106 copies/L) was significantly higher than that in the plasma (mean (1.3± 0.9)× 103 copies/L). There
is a positive association between circulating shope DNA level and 18F-FDG-PET/CT maximum standard uptake value, but no significant
association was observed between plasma shope virus DNA level and VX2 tumor size. Conclusion:Cell-free shope viral DNA in
circulating plasma may be a tumor-marker of VX2 tumor animal model for cancer research, and circulating cell free tumor specific DNA
may be proposed as a novel and early detectable bio-marker as well as an inexpensive and noninvasive assay for cancer management. |
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