| 魏翻艳 李郁 陈必良 董曦文 吴娟 杨红.转录因子FOXM1在卵巢癌细胞中的表达及其对侵袭
转移能力的影响[J].,2014,14(14):2610-2614 |
| 转录因子FOXM1在卵巢癌细胞中的表达及其对侵袭
转移能力的影响 |
| Expression of FOXM1 Transcription Factor and Its Effecton Invasive Capacity in Ovarian Cancer |
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| DOI： |
| 中文关键词: 卵巢癌 FOXM1 MMP9 侵袭 转移 |
| 英文关键词: Ovarian cancer FOXM1 MMP9 Invasion Migration |
| 基金项目:国家自然科学基金项目（81072144） |
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| 摘要点击次数: 863 |
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| 中文摘要: |
| 目的：研究上皮性卵巢癌细胞中FOXM1 的表达，探讨FOXM1 与MMP9 之间的相关性以及与卵巢癌细胞侵袭、转移的关
系。方法：采用Real-time RT-PCR、Western blotting 技术检测pcDNA3.1-FOXM1 和FOXM1-siRNA 分别转染卵巢癌细胞株
HO-8910（低转移）和HO-8910PM（高转移）前后FOXM1 的表达水平，用Transwell 方法检测转染该序列后HO-8910 和
HO-8910PM细胞侵袭能力的改变，并用荧光素酶双报告基因分析技术检测FOXM1 对MMP9 的调控作用。结果：与对照组和空
载组相比，转染了pcDNA3.1-FOXM1 的HO-8910 细胞FOXM1 mRNA、蛋白表达显著升高，而转染了FOXM1-siRNA 的高转移细
胞株HO-8910PMFOXM1 mRNA、蛋白表达显著降低(P<0.05)；相对于空载体组和空白组，pcDNA3.1-FOXM1 转染组细胞的侵袭
能力明显增强，而FOXM1-siRNA转染细胞的侵袭能力明显降低(P<0.05)；FOXM1参与对MMP9 的转录调控作用(P<0.05)。结
论：FOXM1 可能是一个潜在的治疗靶点，通过下调FOXM1 的表达，从而抑制卵巢癌的浸袭和转移。 |
| 英文摘要: |
| Objective: The expression of FOXMI in epithelial ovarian cancer cell was investiageted to learn the relationship
between FOXMI and MMP-9 as well as its role in the invasiveness of ovarian cancer cell.Methods:Rea1-time RT-PCR and Western
blotting were performed to detect the expression levels of FOXM1 before and after overexpression vectors pcDNA3.1-FOXM1 and
FOXM1 siRNA were transfected into HO-8910 and HO-8910PM cells respectively. The change of invasion capacity was evaluated by
Transwell chamber test. The involvement of FOXM1 in regulating MMP9 expression was explored by Dual-luciferase reporter assay
system.Results:After overexpression vectors pcDNA3.1-FOXM1 was transfected into HO-8910, FOXM1 expression at both mRNA and
protein levels were significantly increased and the invasion capacity of ovarian cancer cells was obviously enhanced compared to the
control group (P<0.05). After FOXM1 siRNA was transfected into HO-8910PM, FOXM1 expression at both mRNA and protein levels were
significantly decreased and the invasion capacity of ovarian cancer cells was obviously reduced compared to the control group (P<0.05).
FOXM1 was involved in the transcription regulation of MMP9 (P<0.05).Conclusion:FOXM1 may serve as a promising therapeutic
target for ovarian cancer. The invasion capacity of ovarian cancer cells can be inhibited through down-regulating FOXM1． |
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