| 汪云 李红霞 冯涛 王媛媛 宋波 梅夏齐 梁琦.高浓度藏红花溶液导致大鼠肝损伤中caspase-3、bcl-2、NF-kB表达及意义[J].,2014,14(13):2422-2425 |
| 高浓度藏红花溶液导致大鼠肝损伤中caspase-3、bcl-2、NF-kB表达及意义 |
| Expression of caspase-3、bcl-2 and NF-kB inhepatic tissue of rats with saffron induced hepatic injury |
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| DOI： |
| 中文关键词: 藏红花 肝损伤 半胱氨酸蛋白酶-3 bcl-2 核因子-KB 免疫组化 |
| 英文关键词: saffron hepatic injury Caspase-3 Bcl-2 Nuclear factor-kappa B Immunohistochemistry |
| 基金项目:黑龙江省教育厅基金项目(11551250）;黑龙江省自然科学基金项目( D201076) |
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| 中文摘要: |
| 摘要： 目的 通过研究高浓度藏红花溶液引起肝损伤大鼠肝功能、肝组织病理及肝组织中半胱氨酸蛋白酶-3（caspase-3），bcl-2,NF-kB表达，探讨半胱氨酸蛋白酶-3（caspase-3），bcl-2,NF-kB在高浓度藏红花溶液导致的肝损伤中的作用及机制．方法 清洁级雄性大鼠45只，随机分为3组，每组15只，分别标记为A组(藏红花组)；B组(酒精组，阳性对照)；C组(生理盐水组,阴性对照)。分别给予藏红花高浓度水煎浓缩溶液、60 ％酒精、生理盐水灌胃共6周。第6周末，心腔取血测定ALT、AST。肝组织HE染色，免疫组化方法检测细胞凋亡相关蛋白Caspase-3及调控基因Bcl-2、NF-kB的表达。结果 藏红花组大鼠肝功能ALT、AST升高，肝组织正常结构消失，肝细胞肿胀坏死，部分碎裂，凋亡蛋白caspase-3，bcl-2，NF-kB表达增加（69.6 %± 16.7 % vs 5.3 %±1.6 %；55.4 %±14.5 %vs4.5%±2.8 %；44.1 %±12.6 % vs2.5 %±1.9 %；p <0.05)。结论 高浓度藏红花溶液可以引起大鼠肝损伤，肝组织caspase-3，bcl-2，NF-kB表达增加，细胞凋亡机制参与了藏红花肝损伤过程。 |
| 英文摘要: |
| Abstract:Objective To explore the roles of caspase-3，bcl-2 and NF-kB in saffron induced hepatic injury and their machanisms．Methods A total of 45 male wistar rats were randomly divided into saffron group (n=15)，normal control group(n=15) and model group (alcohol group,n=15) .Rats in the saffron group were fed with saffron 800 mg,kg-1，while rats in the norm al control group and model group were fed with physiological saline and alcohol once a day for 6 month. Blood collection was conducted in all rats at 6 month after last intragastric administration, and levels of serum alanine am inotransferase(ALT ) and aspartate aminotransferase(AST) were detected. Caspase-3,bcl-2 and NF-kB assessed by immunohistochemistry,and the apoptotic index of hepatic cells was also measured.Results Compared to norma1 control group,caspase-3,bcl-2,NF-kB expression in hepatic tissue of rats in saffron group were significantly increased (69.6 %±16.7 % vs 5.3 % ± 1.6 %; 55.4 %±14.5 %vs4.5 %±2.8 %; 44.1 %±12.6 % vs2.5 %±1.9 %; p<0.05). Conclusions High dose saffron can induce hepatic injury. The expression of caspase-3, bcl-2 and NF-kB in hepatic tissue of saffron induced hepatic injury increased and may be play an important role in hepatic injury. |
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