| 晏江 过晓强 夏加增 顾琛.胃癌组织中MEG3 基因甲基化状态及其临床意义[J].,2014,14(12):2289-2292 |
| 胃癌组织中MEG3 基因甲基化状态及其临床意义 |
| Methylation Level of MEG3 Differentially Methylated Regions in GastricCancer and its Clinical Significance |
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| DOI： |
| 中文关键词: 胃癌 MEG3 长链非编码RNA 甲基化 |
| 英文关键词: Gastric cancer MEG3 Long non-coding RNA Methylation |
| 基金项目:江苏省六大人才高峰项目（2011-WSK-013） |
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| 中文摘要: |
| 目的：探讨胃癌中MEG3基因差异性甲基化区域甲基化水平及其与MEG3 表达之间的关系，并分析其临床病理意义。方法：
利用荧光定量PCR 检测38 例胃癌组织及其对应癌旁正常组织中MEG3 的表达水平，并利用甲基化特异性PCR 检测MEG3 基
因差异性甲基化区域的甲基化水平。结果：①胃癌组织中MEG3 表达水平明显低于正常癌旁对照组织（P＜0.05）；②胃癌组织
MEG3差异性甲基化区域的甲基化率（21/38,55.3%）显著高于正常对照组织（10/38，26.3%，P＜0.05）③胃癌组织MEG3差异性甲
基化区域的甲基化率在性别、年龄、发病部位的差异无统计学意义（P＞0.05）；在肿瘤的大小，淋巴结转移，浸润深度上差异有统计
学意义（P＜0.05）。结论：胃癌组织中MEG3 呈低表达水平，其差异性甲基化区域的甲基化与肿瘤的大小，淋巴结转移，浸润深度
有关。 |
| 英文摘要: |
| Objective:To investigate the expression of Maternally expressed gene 3 (MEG3) and clinical significance of
hypermthylation of MEG3 differentially methylated regions (DMR) in gastric cancer tissues.Methods:Quantitative RT-PCR was used to
detect the expression of MEG3 in 38 gastric cancer tissues. Methylation-specific PCR (MSP) was performed to analyze the methylation
statue of MEG3 DMR.Results:① The expression of MEG3 decreased in gastric cancer tissues. ② The methylation rate of MEG3 DMR
was increased significantly in tumor tissues (21/38,55.3%) compared with that in the normal tissues (10/38，26.3%，P＜0.05). ③ The
positive rate of MEG3 DMR methylation was closely related with the tumor size, lymph node metastasis and depth of invasion (P＜0.05).Conclusion:The expression level of MEG3 decreased in gastric cancer tissues, and the aberrant methylation statue of MEG3 DMR was
correlated with the tumor size, lymph node metastasis and depth of invasion. |
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