| 王红芳 宋爱琴 孙立荣 叶俊丽 邢晓明 杨堃 张敏.基因在B 细胞淋巴瘤细胞中的表达及意义[J].,2014,14(12):2242-2246 |
| 基因在B 细胞淋巴瘤细胞中的表达及意义 |
| The Expression and Significance of CHFR in B-cell Lymphoma Cells |
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| DOI： |
| 中文关键词: CHFR基因 Raji 细胞 5-氮杂-2 脱氧胞苷 甲基化 增殖 凋亡 |
| 英文关键词: CHFR gene Raji cell 5-Aza-dC Methylation Proliferation Apoptosis |
| 基金项目:国家自然科学基金项目(31100824)；山东省自然科学基金（Y2008c170）；青岛市自然科学基金项目（2012-1-3-2-（5）-
nsh） |
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| 中文摘要: |
| 目的：探讨基因CHFR在B细胞淋巴瘤Raji 细胞中的表达，以及基因甲基化对Raji 细胞增殖和凋亡中所产生的影
响。方法：体外培养人B细胞淋巴瘤细胞株Raji 细胞，用不同浓度的去甲基化试剂5- 氮杂-2 脱氧胞苷处理Raji 细胞株，通过
RT-PCR 检测CHFR 基因表达水平的变化，通过MS-PCR 检测基因甲基化变化，CCK 法及流式细胞术检测Raji 细胞增殖
及凋亡变化。结果： 基因在Raji 细胞中出现弱表达，经去甲基化试剂处理后基因表达水平增高，随药物浓度增加Raji
细胞的抑制率及凋亡率增高。结论：5-氮杂-2 脱氧胞苷可以恢复基因表达水平，抑制Raji 细胞的增殖，促进凋亡。基
因在细胞增殖起负向调控的作用。 |
| 英文摘要: |
| Objective:To investigate mRNAexpression level of gene in lymphoma cells, the effect of CHFR gene mathylation
and methylation inhibition on Raji cells proliferation and apoptosis.Methods:The human Burkitt's Raji lymphoma cells were cultivated
. Raji cells were treated in different concentration of methylation inhibitor 5 - Aza-2'-deoxycytidine（5-Aza-dC）, then the expression
level change of CHFR gene was detected by RT-PCR and methylation of CHFR gene was analyzed by MS-PCR. The cells proliferation
of Raji cells treated with different concentrations of 5-Aza-dC were analyzed by CCK assay. The apoptosis of Raji cells was analyzed
by flow cytometry.Results:CHFR gene had low-expression in Raji cells. 5-Aza-dC can up-regulate the expression of mRNA by
demethylation. The growth of Raji cells was inhibited and the apoptosis was promoted by 5-Aza-dC.Conclusion:The expression of CHFR
can be recovered by the methylation inhibition. CHFR gene can inhibit growth in Raji cells, and it may play a negative role during
the growth and proliferation. |
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