| 苏元镇 王春波 辛辉 张蕊 田嘉伟 张进松 姚如永.熊果酸对ox-LDL诱导的人脐静脉血管内皮细胞NQO1 表达的影响[J].,2014,14(11):2019-2023 |
| 熊果酸对ox-LDL诱导的人脐静脉血管内皮细胞NQO1 表达的影响 |
| Effect of Ursolic Acid on NQO1 Expression in HUVECsStimulated by ox-LDL |
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| DOI： |
| 中文关键词: 熊果酸 氧化性低密度脂蛋白 醌还原氧化酶1 氧化应激 动脉粥样硬化 |
| 英文关键词: Ursolic acid Ox- LDL NQO1 Oxidative stress Atherosclerosis |
| 基金项目:国家自然科学基金项目（81173593） |
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| 中文摘要: |
| 目的：研究熊果酸对经氧化性低密度脂蛋白（ox-LDL）干预后人脐静脉血管内皮细胞（human umbilical vein endothelial cells,
HUVECs）醌还原氧化酶1 表达的影响,以进一步探讨熊果酸抗动脉粥样硬化的机制。方法：体外培养人脐静脉内皮细胞，进行分
组处理，每组n=5。对照组，不加任何处理；ox-LDL组，加入ox-LDL 培养24h，终浓度为20mg/L；ox-LDL+ 低浓度熊果酸组，先加
入ox-LDL（浓度20mg/L）孕育半小时，然后与熊果酸（浓度1.5umlo/L）共同培养24h；ox-LDL+ 高浓度熊果酸组，先加入ox-LDL
（浓度20mg/L）孕育半小时，然后与熊果酸（浓度4.5滋mlo/L）共同培养24h；采用MTT 试验测定细胞吸光度值,检测熊果酸对
ox-LDL损伤的保护作用，采用RT-PCR 法检测NQO1mRNA的表达，采用Western blot 法检测NQO1蛋白的表达。结果：熊果酸
减弱ox-LDL对HUVECs的损伤作用；ox-LDL 组NQO1mRNA 的表达量(0.624± 0.009)明显高于对照组(0.521± 0.007),P<0.01。熊
果酸呈浓度依赖性的提高NQO1mRNA的表达量（ox-LDL+ 低浓度熊果酸组vs ox-LDL 组：0.722± 0.058 vs 0.624± 0.009, P<0.
01；ox-LDL+ 高浓度熊果酸组vs ox-LDL 组：0.826± 0.059 vs 0.624± 0.009, P<0.01）。ox-LDL 组NQO1 蛋白的表达量(0.624±
0.009)明显高于对照组(0.521± 0.007),P<0.01。熊果酸呈浓度依赖性的提高NQO1 蛋白的表达量（ox-LDL+ 低浓度熊果酸组vs
ox-LDL 组：0.710± 0.058 vs 0.574± 0.024, P<0.01；ox-LDL+ 高浓度熊果酸组vs ox-LDL 组：0.831± 0.034 vs 0.574± 0.024, P<0.
01）。结论：熊果酸可上调ox-LDL诱导的人脐静脉血管内皮细胞NQO1 的表达，表明其可能具有抗氧化应激及抗动脉粥样硬化的
作用。 |
| 英文摘要: |
| Objective: To investigate the effects ofUrsolic acid on NQO1 (Quinone Oxidoreductase1, NQO1)incultured umbilical
vein endothelial cells (HUVECs) stimulated by ox-LDL, so as to approach the mechanism of UA in atherosclerosis.Methods: HUVECs
were cultured in different concentration and divided into four groups（n=5,each）: GroupⅠ, control group without; GroupⅡ, HUVECs
stimulated with ox-LDL (20 mg/L) in endothelial basal medium for 24 hours; GroupⅢ, HUVECs treated with ox- LDL(20m g/L) for an
half hours, and treated with ox- LDL (20 mg/L) and UA (1.5 滋mol/L) in endothelial basal medium for 24 hours; GroupⅣ , HUVECs
treated with ox- LDL(20 mg/L) for an half hours, and treated with ox- LDL (20 mg/L) and UA (4.5umol/L) in endothelial basal medium
for 24 hours. MTT assay was used to determine against HUVECs injury. Expression of NQO1mRNA was determined by RT-PCR.
Expression of NQO1 protein was determined by Western blot.Results:Ursolic acid decreased the effect on reducing the cytotoxicity of
ox- LDL. Expression of NQO1mRNA was significantly highter in groupⅡ（0.624± 0.009）than that in groupⅠ（0.521± 0.007）, P<0.01.
Ursolic acid dose-dependenly increased expression of NQO1 mRNA (GroupⅢ vs GroupⅡ : 0.722± 0.058 vs 0.624± 0.009, P<0.01;
GroupⅣ vs GroupⅡ : 0.826 ± 0.059 vs 0.624 ± 0.009, P<0.01). Expression of NQO1 Protein was significantly highter in groupⅡ
（0.574± 0.024）than that in groupⅠ(0.438± 0.039), P<0.01. Ursolic acid dose-dependenly increased expression of NQO1 Protein(Group
Ⅲ vs GroupⅡ : 0.710± 0.058 vs 0.574± 0.024, P<0.01; GroupⅣ vs GroupⅡ : 0.831± 0.034 vs 0.574± 0.024, P<0.01). Conclusion:NQO1 expression in ox-LDL- treated HUVECs could be increased by UA which suggests that UA may attenuate atherosclerosis by reducing
ox-LDL-induced oxidative stress responses. |
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