| 倪海波 张宝乐 任庆先 续继军 高殿帅 虞正权.大鼠C6胶质瘤细胞中gdnf基因启动子Ⅰ区组蛋白高乙酰化
可能参与了其高转录调控过程[J].,2014,14(11):2015-2018 |
| 大鼠C6胶质瘤细胞中gdnf基因启动子Ⅰ区组蛋白高乙酰化
可能参与了其高转录调控过程 |
| Histone Hyperacetylation of gndf Promoter RegionⅠMay be Involved in itsHigh Expression in Rat C6 Glioma Cells |
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| DOI： |
| 中文关键词: gdnf 启动子 组蛋白H3 乙酰化 胶质瘤 |
| 英文关键词: Gdnf Promoter Histone H3 Acetylation Glioma |
| 基金项目:国家自然科学基金项目（31271358）；江苏省自然科学青年基金（SBK201341565）；江苏省博士后基金（1301068C）；
枣庄市医药卫生科技发展计划项目（2013005）；枣庄市科学技术发展计划项目（201361） |
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| 中文摘要: |
| 目的：探讨大鼠C6 胶质瘤细胞中gdnf基因高转录与其启动子Ⅰ区组蛋白乙酰化的关系。方法：应用Real-time PCR 和
ChIP-PCR 技术分别检测了大鼠正常星形胶质细胞和C6 胶质瘤细胞中gdnf基因mRNA 的表达水平以及其启动子Ⅰ区组蛋白
H3K9 的乙酰化程度；利用Real-time PCR 技术，检测了不同浓度的组蛋白乙酰基转移酶抑制剂姜黄素（Curcumin）或去乙酰化酶
抑制剂曲古抑菌素A（TSA）处理对C6 胶质瘤细胞中gdnf基因mRNA表达的影响。结果：较之正常星形胶质细胞，C6 胶质瘤细
胞中gdnf基因mRNA 的表达量极显著增高（P<0.01），并且其启动子Ⅰ区H3K9的乙酰化水平也显著升高（P<0.05）。C6 胶质瘤细
胞经Curcumin 处理24 h后， gdnf基因mRNA的表达量随药物浓度的升高而降低，且100 滋mol/L 作用浓度时其表达量下降了
74.17 %（P<0.001）；相反，TSA 处理后gdnf基因mRNA 的表达量呈上升趋势，且200nmol/L 组其表达量约上升145.35 %
（P<0.05）。结论：在大鼠C6胶质瘤细胞中gdnf基因启动子Ⅰ区H3K9 发生了高乙酰化修饰，这种修饰可能是其高转录的原因。 |
| 英文摘要: |
| Objective:To investigate the relationship between gndf mRNA expression and histone acetylation of the promoter regionⅠ
in rat C6 glioma cells. Methods: Real-time PCR and ChIP-PCR techniques were used respectively to detect the expression level of
gndf mRNA and acetylation status of histone H3K9 in the promoter regionⅠ in normal rat astrocytes and C6 glioma cells; After treating
with varying concentrations of histone acetyltransferase inhibitor Curcumin or deacetylase inhibitor trichostatinA (TSA) in C6 glioma
cells, the expression of gndf mRNAwas detected by real-time PCR.Results:Compared with that in the normal astrocytes, the gdnf mRNA
expression increased significantly in C6 glioma cells (P<0.01), and the acetylation of H3K9 also enhanced in promoterⅠregion significantly
(P<0.05). At 24 h after treatment with Curcumin, the expression of mRNA decreased in concentration-dependent manner in
C6 glioma cells, and about 74.17%decreased was observed in 100 umol/Lgroup (P<0.001); In contrary, gndf mRNA expression rase after
TSA treatment, and a near-maximum increase of 145.35%was observed in 200 nmol/L group (P<0.05). Conclusion:H3K9 hyperacetylation
occurred in gndf promoter regionⅠ, which may be the cause of high gdnf transcription in rat C6 glioma cells. |
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