| 胡静1 马力天2,5 闫平4 魏忠海4 张莹1 张平5 田兆林5
郑瑾2,3△ 石梅1△ 魏丽春1△.康莱特联合顺铂对宫颈癌SiHa细胞增殖和凋亡的影响*[J].,2014,14(10):1865-1870 |
| 康莱特联合顺铂对宫颈癌SiHa细胞增殖和凋亡的影响* |
| Effect of KLT combined with DDP on the Proliferationand Apoptosis of SiHa cell* |
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| DOI： |
| 中文关键词: 康莱特 顺铂 Siha 细胞 增殖 凋亡 |
| 英文关键词: KLT DDP SiHa cell Proliferation Apoptosis |
| 基金项目:国家自然科学基金面上项目(81072973)；国家自然科学基金青年科学基金项目(81201749)； |
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| 中文摘要: |
| 摘要目的：通过康莱特联合顺铂对宫颈癌SiHa 细胞增殖和凋亡的影响，探讨其作用机制。方法：体外培养宫颈癌Siha细胞，分别
将康莱特( 浓度为1，2，4，6，8 mg/mL)，顺铂(浓度梯度为1.5，3，6，9，12 μg/mL)，单独作用于宫颈癌SiHa 细胞,加药24h、48h 用噻唑
蓝(MTT 法)检测细胞增殖情况。用流式细胞术检测康莱特组和顺铂组细胞24h 凋亡率，选取合适的药物浓度(康莱特6 mg/mL，顺
铂3 μg/mL)，进行联合用药，加药24h、48h用MTT 法检测细胞增殖情况，用流式细胞术检测24h 细胞凋亡率。结果：①MTT法显
示加药后两组的24h、48h，宫颈癌SiHa 细胞的抑制率均高于对照组（P<0.05），并且在一定程度上呈浓度和时间依赖性。②联合用
药时，细胞的抑制率和凋亡率要显著高于单独用药（P<0.01）。结论：康莱特、顺铂单独或联合作用均能抑制SiHa 细胞的增殖，促进
其凋亡，且康莱特联合顺铂的作用要显著高于单独用药，康莱特与化疗药物联合使用可提高肿瘤细胞对化疗药物的敏感性。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the effect of KLT combined with DDP on the proliferation and apoptosis of SiHa cell.
Methods：Different concentrations of KLT injection (1, 2, 4, 6, 8 mg/ml)and DDP(1.5, 3, 6, 9, 12 μg/mL)were solo applied to SiHa cells.
Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. The appropriate drug concentration (KLT 6
mg/ml，DDP 3 μg/mL)were selected. Then, KLT combined with DDP injection was applied to SiHa cells. Cell proliferation was
measured by MTT, cell apoptosis was detected by flow cytometry.Results： ① Cell proliferation were dramatically inhibited by KLT
injection (25，50，100，150，200 μg/mL)and DDP (1.5, 3, 6, 9, 12 μg/mL)at 24 h,48 h (P<0.05). In addition, Cell proliferation were
dramatically inhibited by injected KLT combined with DDP. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in
SiHa cells treated by KLT combined with DDP were higher than the monotherapy. Conclusion：DDP, KLT and DDP combined with KLT
can inhibit the proliferation, accelerate the apoptosis in SiHa cells. What's more, DDP combined with KLT have synergistic effect on the
apoptosis in SiHa cells. |
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