| 刘德勇1 张庆丽1 王海伦1 彭和人1 谢海龙2△.DADS对人小细胞肺癌NCI-H446 细胞系增殖抑制的研究*[J].,2014,14(8):1460-1463 |
| DADS对人小细胞肺癌NCI-H446 细胞系增殖抑制的研究* |
| Proliferation Inhibited in NCI-H440 Cells with DADS* |
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| DOI： |
| 中文关键词: 二烯丙基二硫 小细胞肺癌 MTT |
| 英文关键词: DADS Small cell lung cancer MTT |
| 基金项目:湖南省教育厅优秀青年基金项目（04B057） |
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| 中文摘要: |
| 摘要目的：研究二烯丙基二硫（diallyl disulfide，DADS）对人小细胞肺癌NCI-H446细胞增殖的抑制作用，并探讨其作用机制。方
法：体外培养NCI-H446 细胞，采用MTT、细胞计数实验方法检测DADS 抑制NCI-H446 细胞增殖；通过HE 染色和AO-EB 荧光
染色方法，观察DADS 处理后NCI-H446 细胞的形态学改变。结果：MTT 结果显示：DADS作用于NCI-H446 细胞48 h后，代谢
MTT 的能力明显降低，显示出较强的细胞毒性反应，IC50值介于20～40 滋g/ml之间。细胞计数结果表明：DADS 作用于NCI-H446
细胞后，随DADS 浓度增加NCI-H446 细胞倍增时间延长。HE染色显示：NCI-H446 细胞经DADS处理24 h后，与对照组相比，细
胞体积变小，胞浆丰富,细胞核变小，染色变淡。AO-EB 荧光染色显示：NCI-H446 细胞经DADS处理24 h后，与对照组相比，细胞
皱缩、呈圆形，胞质黄色或橘红色，细胞核或细胞质内可见致密浓染的黄绿色或橘红色荧光，并可见橘红色碎片且随DADS 浓度
增加，随DADS浓度增加细胞密度逐渐减少。结论：DADS 能抑制体外培养的NCI-H446 细胞增殖，作用效果与药物浓度及作用
时间相关。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effect and the mechanism of DADS on the growth of human small cell lung cancer
NCI-H446 cells in vitro .Methods: NCI-H446 cell lines were cultured . Cell proliferation inhibition was measured by MTT assay
and cell count experiments. Cell morphologic change was observed by HE staining and AO-EB fluorescence staining. Results:MTT assay
showed that the ability of the metabolic MTT reduced significantly and it had a strong cytotoxic response after cultured 48 hours by
DADS. IC50 values located between 20 ~ 40 滋g/ml. Cell count experiments showed that NCI-H446 cells average doubling time extended
after exposure to different concentration DADS. HE staining showed that cell volumes and nucleus become smaller while cytoplasm
became abundant and stain hard 24 hours after H446 cellular exposure to DADS. AO-EB fluorescence staining showed that the cells
morphoous and colour had huge changed that dealed with DADS. Conclusion:DADS could markedly inhibit the proliferation of
NCI-H446 and the effect depended on the concentration and the time of DADS. |
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