| 陈璐 王博 姚蜜蜜 蔡中华.基于COMSTAT软件对微生物被膜进行定量分析的方法与意义[J].,2014,14(7):1201-1206 |
| 基于COMSTAT软件对微生物被膜进行定量分析的方法与意义 |
| Construction and Significance of Quantitative Analysis of MicrobialBiofilmBased on COMSTAT Program |
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| DOI： |
| 中文关键词: COMSTAT 软件 微生物被膜 定量分析 葡萄球菌 |
| 英文关键词: COMSTAT software Microbial biofilm Quantitative analysis |
| 基金项目:国家973前期专项(2012CB426504)；国家自然科学基金项目(41106087)；国家海洋公益性项目（201205020-09；20130502）；
广东省自然科学基金项目（S2012010010855） |
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| 中文摘要: |
| 目的：微生物被膜是一种具有协调性、功能性和高度结构性的膜状复合物,它可以为微生物提供良好的生存环境，免受外界
因素的干扰。研究发现，具有产生微生物被膜能力的细菌治病性明显增强，而现今缺乏一种分析微生物被膜的有效手段。本文探
讨利用COMSTAT软件对微生物被膜进行定量分析的方法和意义，为对微生物被膜定性定量分析提供支持手段，从而为研究微
生物被膜致病性提供方法理论基础。方法：以葡萄球菌为研究模型，利用激光扫描共聚焦显微镜成像技术，结合COMSTAT 微生
物被膜分析软件对微生物被膜的单位面积生物量、基质覆盖率、平均厚度、粗糙系数等方面进行定量分析，研究了该葡萄球菌的
生物被膜生长变化过程，并考察了抗生素对其生物被膜的抑制作用。结果：在葡萄球菌生物被膜生长过程中，生物量、平均厚度以
及平均扩散距离等结构指标数值都有明显增加，而粗糙度和表面积与生物量比值呈现降低趋势，表明了微生物被膜由发生向成
熟的转化过程。与此同时，经10ug/mL和100ug/mL的卡那霉素处理得到的葡萄球菌微生物被膜生长受到明显抑制，且随着卡那
霉素的浓度增加，抑制效果随之增加。结论：本文运用COMSTAT 软件的分析方法首次从生物量、平均厚度等结构指标数值的角
度描述了葡萄球菌生物被膜，从而有效评价微生物被膜发生、发展、成熟以及崩解的生长过程。该技术在研究微生物被膜形成的
理论机制方面存在潜在价值，可以为研究微生物被膜治病性提供理论基础，具有理论指导意义。 |
| 英文摘要: |
| Objective:Microbial biofilms are coordinated, functional and highly structured community. Under the protection of
biofilms, bacteria can be free fromthe interference of external factors. Studies found that biofilms should be responsible for some chronic
and incurable diseases caused by pathogenic bacteria. Therefore, a pressing matter is searching for effective methods for the analysis of
bacterial biofilm. We investigate the construction and significance of quantitative analysis of microbial biofilmbased on COMSTAT program
and aim to provide the method for qualitative and quantitative analysis, as well as theoretical basis of biofilm of pathogenic
microorganisms.Methods: According to confocal microscopy imaging combined with COMSTAT analysis, Staphylococcus sp. was
selected as the object of study. Features of Bio-volume, average thickness, average diffusion distance and etc. were determined to characterize
the growth process of biofilmand inhibition of kanamycin.Results:During the biofilmprocess of Staphylococcus sp., feature index
of bio-volume, average thickness and average diffusion distance are significantly increased, while the roughness coefficient and surface to
volume ratio showed a decreasing trend, indicated the maturation process of biofilm. Also, biofilm structure of . was
inhibited by Kanamycin in both 10 ug/ml and 100 ug/ml treatment.Conclusion:The structural index of . is well
characterized, and the occurrence, development, maturation and dissolution process of biofilm are well evaluated based on quantitative
analysis of COMSTAT program. There existed potential value on the study of theory mechanism of microbial biofilm formation, and
could provide theoretical basis for the research on microbial biofilmtreatment. |
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