| 吴建红 张 飞 赵 炜 张 琦 刘海涛
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夏术阶.免疫重建荷人膀胱癌 -SCID 鼠模型的建立 *[J].,2014,14(6):1033-1035 |
| 免疫重建荷人膀胱癌 -SCID 鼠模型的建立 * |
| Establishment and Identification of HU-PBL-SCID Mice Model of HumanBladder Cancer Model* |
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| DOI： |
| 中文关键词: 膀胱癌 SCID 鼠 动物模型 免疫重建 |
| 英文关键词: Bladder tumor SCID mouse Animal model Immune reconstruction |
| 基金项目:上海市医学领先专业重点学科建设基金 （061125 ） |
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| 中文摘要: |
| 摘要 目的：探讨建立稳定的荷人膀胱癌 SCID 鼠人化复合型的方法，为在人免疫重建荷人膀胱肿瘤 SCID 鼠型检测重组
BCG 的免疫刺激和免疫保护中打下基础。方法： 经 SCID 鼠腹腔内注射人 PBL、 皮下接种 RT4 膀胱癌细胞， 于接种后 4 周检测
SCID 鼠体内人免疫细咆水平(IgG 和 CD
3+
、 CD
4+
、 CD
8+
T 细胞)及脾脏重量， 观察皮下成瘤潜伏期及成瘤率。结果： 型组 100％ 成
瘤，病理类型为移行细胞癌； 检测 SCID 鼠血中人 IgG 均一定水平存在， 与对照组间差异有统计学意义(P<0.01)； 人 CD
3+
、 CD
4+
、
CD
8+
T 细胞在鼠血及脾中均有表达， 而对照组均未检出(P<0.05)； 4 周鼠脾重平均(178.9± 45.2)mg。较对照组(40.6± 14.8)mg 差异
有统计学意义(P<0.01)。结论： 采用腹腔注射人 PBL、 皮下接种 RT4 膀胱癌细胞的方法可以有效地建立人化荷人膀胱癌 SCID 鼠
型， 较好地拟人浸润性膀胱癌体内生物学特性，是膀胱癌免疫基因治疗的较理想型。 |
| 英文摘要: |
| ABSTRACT Objective：To establisha human bladder cancer model in Hu-PBL-SCID mice. Methods:The immunological features
of mice were evaluated after intra-peritoneal injection of hu-PBL and subcutaneous implantation of human RT4 cells.Results: 16 mice
had pathologically transitional cell carcinoma tumor in group one. The level of the human IN. Was gradually elevated in all the humanized mice following the time of PBL transplantation prolonging until 4th week on, and was significantly higher than that in control mice
(P<0.01). The different level of human CD
3+
, CD
4+
, CD
8+
T cells among two groups was statistically significant(P<0.05). Furthermore, human CD
3+
, CD
4+
, CD
8+
T cells in mice blood and spleen cells could be still detected at 4th week. The weight of spleen was averagely
(178.9 ± 45.2) mg in group one at 4th week, and had statistical significance compared with the control group (P<0.01).Conclusion: Humanized SCID mice model can he successfully established via intraperitoneally human peripheral blood cells into SCID mice. The model
simulat- ed the real biological behavior of human bladder invasive carcinoma very well. |
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