| 赵德根 王建校 刘旭 徐涛 顾永清.小干扰RNA的研究进展[J].,2014,14(4):792-794 |
| 小干扰RNA的研究进展 |
| Advance of the Study of siRNA |
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| DOI： |
| 中文关键词: siRNA 干扰机制 设计原则 缺点 |
| 英文关键词: Small interfering RNA Interference mechanisms Regulation of design Shortcomings |
| 基金项目:国家自然科学基金项目（30960093；31160183；31270894） |
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| 中文摘要: |
| RNA干扰(RNA interference, RNAi)是近年发展起来的一种新技术。RNAi是指通过外源性或内源性的双链RNA 在体内诱
导靶基因mRNA产生特异性降解,进而引起不同水平的基因沉默，其效应分子主要是小干扰RNA(siRNA）。siRNA是生物界普遍
存在的一种抵御外来基因和病毒感染的基因调控方式，也是一种重要的研究工具。大量的研究工作致力于设计合理的siRNA 片
段用于基因功能研究，并将其作为一种治疗方法用于肿瘤、病毒性疾病等基因治疗以及药物靶向研究。因此本文对siRNA的作用
机制、设计原则及其在临床应用中的缺点和解决方法进行综述。 |
| 英文摘要: |
| RNA interference (RNAi) is a newly developed technology. It is the different levels of gene silencing induced by
specific degradation of targeted genes in vivo , and both exogenous and endogenous double- stranded RNAs could induce the specific
degradation, which was realized mainly by Small interfering RNA (siRNA). siRNA was commonly exists in the nature as a gene
regulation way to prevent alien gene and viral infection. And SiRNA is becoming an important investigating tool. A lot of studies were
devoted to the effective sequence design of siRNA to study gene's function. SiRNA is regarded as an effective therapy for cancer, viral
disease and study for drug targeting. This paper focuses on the interference mechanisms, design regulations, shortcomings in clinical
treatment and solutions of siRNA. |
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