| 徐爱晶 陈志红 田飞 阎丽华 朱伟.IL-10 基因对糖尿病大鼠胰岛beta细胞凋亡的保护作用[J].,2014,14(4):671-675 |
| IL-10 基因对糖尿病大鼠胰岛beta细胞凋亡的保护作用 |
| The Protective Effect of IL-10 Gene on Pancreatic Beta-cellin STZ-induced Diabetic Rats |
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| DOI： |
| 中文关键词: 腺病毒 IL-10 胰岛细胞 凋亡 |
| 英文关键词: Adenovirus IL-10 Islet beta cells Apoptosis |
| 基金项目:广东省自然科学基金项目（S2012040006330）；广东省科技计划项目（2012B031800016） |
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| 中文摘要: |
| 目的：研究白细胞介素10（IL-10）基因对链脲佐菌素(STZ)诱导的糖尿病大鼠胰腺炎症浸润程度及胰腺组织中Bcl-2 及Bax
表达的影响。方法：建立链脲佐菌素性糖尿病模型,腺病毒介导的IL-10 基因(Ad-mIL-10)腹腔注射。检测大鼠空腹血糖值；免疫组
织化学法观察胰腺炎症浸润程度；TUNEL法检测胰岛细胞凋亡；免疫组化方法观察Ad-mIL-10 对实验性糖尿病大鼠胰岛凋亡调
控基因Bax 和Bcl-2 表达的影响。结果：Ad-mIL-10 腹腔注射糖尿病发病率低,平均血糖水平低，可以降低胰腺炎症浸润程度，减
少胰岛细胞凋亡。给予Ad-mIL-10 后大鼠Bax 基因的表达明显下降, Bcl-2 与Bax 的比值明显增加。结论：IL-10基因对实验性糖
尿病大鼠有降血糖作用,减少胰岛细胞凋亡，与调节Bcl-2 与Bax 基因的表达有关。 |
| 英文摘要: |
| Objective:To investigate the effect of adenovirus mediated interleukin-10 gene on streptozotocin (STZ)-induced type 1
diabetic mellitus (T1DM) in SD rats, and to investigate the expression of Bcl-2 and Bax.Methods:Forty male SD mice aged fromfour to
five weeks were randomly divided into 4 groups. Group1 (vacuity group) was without special disposal; Group 2 was diabetes mellitus
control goup; Group 3 received the intraperitoneal injection of adenovirus vector mediated empty green fluorescence protein (Ad-eGFP);
Group 4 received intraperitoneal injection of adenovirus vector mediated IL-10 gene (0.2 mL). Group2, 3 and 4 received intraperitoneal
injection of stz 50 mg/kg in order to induce diabetic mellitus of SD mice. Pancreatic histology was measured for determination of insulitis
grades. Apoptosis was measured by using a TUNEL assay. Expressions of Bcl-2 and Bax were estimated by immunohistochemistry
analysis.Results:Compared with that in the control group, Ad-mIL-10 group had lower incidence in diabetes and lower degree of
average blood glucose; Pancrea pathology showed that inflammatory infiltration was lighter and beta-cell apoptosis decreased. Decreased
expression of Bax and incresed rate of Bcl-2/Bax was observed in the Ad-mIL-10 group.Conclusion:IL-10 gene therapie is able to
decrease the average blood glucose and exhibits anti-apoptotic ability. The expressions of Bax and Bcl-2 in the pancreas showed positive
correlation with apoptosis. |
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