| 侯丽丽 杨志杰 高慧棋 路宇 谢耀萍 刘佳.高压氧在放射性核素治疗大鼠种植癌后乏氧研究[J].,2014,14(4):639-642 |
| 高压氧在放射性核素治疗大鼠种植癌后乏氧研究 |
| The Hypoxic Imaging of Combinating Hyperbaric Oxygen Therapywith Radionuclide on Rats with Implanted Tumor |
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| DOI： |
| 中文关键词: 高压氧 放射性核素显像 HL91 胶体磷[32P]酸铬 |
| 英文关键词: Hyperbaric oxygen Radionuclide imaging HL91 Colloidal chromic 32phosphate |
| 基金项目:黑龙江省教育厅科学技术研究项目（11531189） |
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| 中文摘要: |
| 目的：研究大鼠种植癌在高压氧（HBO）干预及放射治疗前后99mTc-HL91 乏氧显像的变化，并探讨其与病理学改变之间的
关系，为HBO联合放射性药物对恶性肿瘤治疗效果提供实验支持。方法：建立肿瘤株walker-256 细胞大鼠皮下种植癌的动物模
型，60 只荷瘤大鼠随机分为四组：对照组，高压氧（HBO）组、胶体磷[32P] 酸铬和HBO+ 胶体磷[32P] 酸铬组。尾静脉注射
99mTc-HL91 37MBq(0.1 mL)，4 h后利用SPECT 显像，计算肿瘤组织与对侧相应部位放射性计数比值（T/NT），显像当日游标卡尺
测量肿瘤最大长径（a）和最大垂直横径（b），计算肿瘤体积以及治疗后不同时间的肿瘤生长率（f）。最后一次显像结束后处死全部
模型大鼠，取肿瘤组织制成病理切片，观察各组大鼠肿瘤细胞的凋亡情况。比较实验各组T/NT 与肿瘤生长率（f）以及凋亡的关
系。结果：肿瘤99mTc-HL91 显像良好，肿瘤部位与对侧相应部位具有较高的放射性计数比。治疗后大鼠肿瘤的乏氧区域及肿瘤
体积均减少，以HBO+ 胶体磷[32P]酸铬组为著，T/NT 与f 呈正相关；大鼠肿瘤的细胞凋亡数明显高于对照组，以HBO+ 胶体磷
[32P]酸铬组增加明显，治疗后T/NT 与大鼠肿瘤细胞的凋亡数呈负相关。结论：HBO在放射性核素治疗大鼠种植癌中起到协同作
用，通过99mTc-HL91 乏氧显像观察HBO干预后胶体磷[32P]酸铬治疗肿瘤效果，从而为二者联合在肿瘤治疗的应用提供依据。 |
| 英文摘要: |
| Objective:To observe the changes of 99mTc-HL91 imaging in implanted tumor on mice before and after hyperbaric
oxygen therapy with radiotherapy, and investigate the relationship with pathology to provide the basis for the evaluation of clinical tumor
treatment with 99mTc-HL91 imaging.Methods:Sixty tumor-bearing walker-256 mice were randomly assigned into an HBO group, a
Colloidal chromic 32phosphate group, an HBO and Colloidal chromic 32phosphate group and the control group (n=15). Mice were killed
after injection 37MBq (0.1 mL)99mTc-HL91 for SPECT planar imaging and the radioactive count ratio (T/N) calculation. The maximum
m diameter (a), the maximum vertical diameter (b), tumor volume and the tumor growth rate (f) after the treatment were calculated and
the tumor pathological was observed for apoptosis. T / NT, tumor growth rate (f) was compared in some experimental groups and their
relationships to apoptosis.Results:The 99mTc-HL91 imaging was well and the ratio of radioactive counts between tumor tissue and
contralateral sites was high. Region of rat hypoxia region and tumor volume after treatment was reduced, especially in the 32P+HBO
group, and the T/NT of hypoxia imaging was positively correlated with tumor growth. Tumor cells apoptosis after treatment was
significantly higher than that in the control group, especially in 32P+HBO group, the T/NT was negative correlated with cells apoptosis.Conclusion:99mTc-HL91 hypoxia imaging is effective way which is non-invasive monitoring the state of tumor hypoxia. It can predict
the effect of 32P-colloid after the intervention of HBO, thus provides the basis of their combination in cancer therapy. |
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