| 卢强 闫小龙 黄立军 韩勇 张新伟 李小飞.高压电场不可逆电穿孔诱发A549 肺癌细胞凋亡的实验研究[J].,2014,14(3):425-428 |
| 高压电场不可逆电穿孔诱发A549 肺癌细胞凋亡的实验研究 |
| Empirical Study of A549 Lung Cancer Cells Apoptosis Inducedby Irreversible Electroporation of High Voltage Electric Field |
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| DOI： |
| 中文关键词: 高压电场 不可逆电穿孔 A549 细胞 细胞抑制 细胞凋亡 |
| 英文关键词: High voltage electric field Irreversible electroporation A549 lung cancer cells Cell inhibition Apoptosis |
| 基金项目:陕西省自然科学基金项目（2012JM4022）；唐都医院创新科技发展基金资助项目（2012CXTS005）；唐都医院骨干人才基金资助项目 |
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| 中文摘要: |
| 目的：不可逆电穿孔是治疗肿瘤的新兴技术，本文探讨高压电场引起的不可逆电穿孔诱发A549 肺癌细胞凋亡的特点。方
法：选择处于生长周期的A549细胞，共分为A-G7 个组进行研究，其中A组为不施加电场的空白对照组，B-G 组为实验组，B 组
施加500 V/cm 强度高压电场，G 组施加1750 V/cm的高压电场，BG 组之间各组的高压电场强度间隔为250 V/cm。采取细胞抑制
实验、不可逆电穿孔示踪实验、细胞凋亡实验，检验A549 细胞细胞凋亡与电场强度的关系。结果：①各实验组与对照组、各实验组
之间的细胞抑制率，均存在显著性差异(P<0.05)；② 电场强度≥ 1000V/cm时，细胞不可逆电穿孔率明显增加，有统计学意义(P<0.05)；电
场强度≥ 1500 V/cm 时，细胞不可逆电穿孔率增加不明显，无统计学意义(P＞0.05)；③ 电场强度≥ 1250 V/cm时，细胞早期凋亡率
明显增加，有统计学意义(P<0.05)。结论：高压电场不可逆电穿孔诱发A549 肺癌细胞发生早期凋亡的强度为1250 V/cm，发生晚期
凋亡的强度为1500 V/cm，且凋亡率随着电场强度的增加持续升高。这对于高压电场不可逆电穿孔效应引起的肿瘤细胞凋亡机制
的研究具有重要意义。 |
| 英文摘要: |
| Objective:Irreversible Electroporation is the treatment of tumors of emerging technologies. Evaluatesthe characterof
the apoptosis of A549 lung cancer cells induced by irreversible electroporation of high voltage electric field had been studied in this article.Methods:Selecting A549 lung carcinoma cells being in growth cycle. There were seven groups named A to G, and the control group
is A without electric field. Exerting high voltage electric field of 500 V/cm to B and 1750 V/cm to G. The distance of high voltage elecric
field is 250 V/cm. Cell inhibition assay, tracing the irreversible electroporation and apoptosis assays were taken to evaluate the relationhip
between the apoptosis of A549 lung carcinoma cells with high voltage electric field.Results:① Significant differences of cell inhibition
rate were found between each subgroup (P<0.05). ② Significant differences of irreversible electroporation rate were found when the
electric field strength increased upon 1000 V/cm(P<0.05). No significant differences of irreversible electroporation rate were found when
the electric field strength increased upon 1500 V/cm (P>0.05). ③ Apoptosis in prophase increased significantly when the electric field
strength was greater than 1250 V/cm (P<0.05).Conclusion:Apoptosis in prophase appeared at the electric field strength of 1250 V/cm
with irreversible electroporation. Apoptosis in advanced stage appeared at the electric field strength of 1500 V/cm with irreversible
electroporation, and the apoptosis rate continuing to increase along with the electric field strength increasing. It was important that high
voltage field induced the apoptosis of A549 lung carcinoma cells. |
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