| 刘倩 李凤兰 孙美娜 陈明子 赵汉东 李晖.低硒心肌损伤与钾离子通道蛋白的关系研究[J].,2014,14(3):411-413 |
| 低硒心肌损伤与钾离子通道蛋白的关系研究 |
| Study of Relationship between Cardiomyocytes Injury Induced bySelenium-deficiency and PotassiumChannel Protein |
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| DOI： |
| 中文关键词: 低硒 心肌损伤 钾通道蛋白 |
| 英文关键词: Seleniumdeficiency Cardiomyocytes injury Potassiumchannel protein |
| 基金项目:国家自然科学基金项目（30771863；81172616） |
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| 中文摘要: |
| 目的：心肌上的离子通道蛋白与心肌损伤有很大的关系，本研究通过低硒喂养对C57BL/6小鼠心肌组织损伤的影响及其对
钾通道蛋白的改变。方法：将实验小鼠分为4 组：对照组，低硒30 天组，低硒90 天组和低硒180 天组。采用低硒饲料（硒含量
0.0045 ug/g）喂养的方法建立低硒小鼠模型，对照组给予正常饲料(硒含量0.256 ug/g)，与低硒组同时喂养；硒含量的测定和HE
染色方法观察心肌损伤情况，Western Blotting方法检测其钾通道蛋白的表达。结果：低硒饲料喂养小鼠的心脏硒含量与正常饲料
喂养的硒含量相比明显降低（P＜0.01）；并出现轻微的心肌损伤，钾通道蛋白的表达量在低硒30 天组，低硒90 天组和低硒180 天
组下调（P＜0.01）。结论：成功建立低硒小鼠模型，低硒能引起小鼠心肌损伤，这种改变可能有心脏的钾通道蛋白的表达水平有关。 |
| 英文摘要: |
| Objective:Many studies showed that ardiomyocytes injury had related to ion channel, to study the effects of melatonin
on heart tissue in C57BL/6 mice caused by low seleniumdiet and discuss the possible mechanism.Methods:The mice were divided into
four group, with three treatment groups fed with selenium-deficient diet (0.0045 ug/g) for control group, 30 day low-selenium, 90 day
low-selenium, 180 day low-selenium. Then selenium and heart tissues were examined in mice. Western Blotting was employed to
identify the down-stream protein of potassium channel protein expression level.Results:Compared with the control group, the selenium
levels of the 30 days, 90 days and 180 days seleniumdeficiency mice were reduce (P<0.01) and there was cardiomyocytes injury. And the
expression of potassium channel protein in 30 days, 90 days and 180 days selenium deficiency mice was also down-regulated (P<0.05).Conclusion:We established a selenium-deficient mice model successful. Low selenium can caused cardiomyocytes injury and these
changes might be caused by the level of potassiumchannel protein. |
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