| 马美娟李妍△ 张英梅闫浩刘敏张乐
马颖苑健胡建华纪兆乐张荣庆李聪叶王海昌.不同程度内质网应激对巨噬细胞自噬的影响*[J].,2014,14(2):209-213 |
| 不同程度内质网应激对巨噬细胞自噬的影响* |
| Effects of the Different Degree of the Endoplasmic ReticulumStress onMacrophage's Autophagy* |
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| DOI： |
| 中文关键词: 巨噬细胞 内质网应激 自噬 GRP78 P62 |
| 英文关键词: Macrophage Endoplasmic reticulumstress Autophagy GRP78 P62 |
| 基金项目:国家自然科学基金项目（81170184）；陕西省社会发展公关计划课题（2012SF2-02-3） |
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| 中文摘要: |
| 摘要目的：采用ox-LDL诱导巨噬细胞泡沫化模型，通过不同剂量衣霉素诱导巨噬细胞不同程度内质网应激，观察对其自噬的影
响。方法：不同剂量衣霉素作用于小鼠巨噬细胞系RAW264.7，通过TUNEL染色检测其凋亡率，Western blot检测内质网应激蛋白
GRP78，以及自噬标志蛋白P62 的表达水平。结果：与ox-LDL组和大剂量衣霉素组相比，小剂量衣霉素组可以显著减少巨噬细胞
的凋亡（P<0.01）；与ox-LDL 组相比，小剂量衣霉素组上调内质网应激蛋白GRP78表达的同时，自噬标志蛋白P62 适度下降（P<
0.01）；大剂量衣霉素组更为显著地上调了内质网应激蛋白GRP78 表达，但同时自噬标志蛋白P62 也显著增加（P<0.01）。结论：小
剂量衣霉素引起一定程度的内质网应激，可以激活适度的自噬，从而减少巨噬细胞的凋亡，可能有助于降低动脉粥样硬化的程
度。 |
| 英文摘要: |
| ABSTRACT Objective：By using ox-LDL-induced macrophage foam cell model, we used different doses of tunicamycin to induce
different degree of the endoplasmic reticulum stress, to observe the effects of it on autophagy.Methods： Different doses of tunicamycin
were added to macrophage RAW264.7. The apoptosis rate was detected with TUNEL method. And the expression level of the
endoplasmic reticulum stress marker protein GRP78 and autophagy marker protein P62 were detected by western blotting.
Results：Compared with the ox-LDL group and the high-dose tunicamycin group, low-dose of tunicamycin significantly reduced the apoptosis of
macrophage (P<0.01); compared with ox-LDL group, the endoplasmic reticulumstress marker protein GRP78 expression was remarkable
increased in low-dose tunicamycin group; meanwhile, the autophagy marker protein P62 moderately decreased (P<0.01); in the high-dose
tunicamycin group, there was more significant increase in the endoplasmic reticulum stress marker protein GRP78 expression, however
the autophagy marker protein P62 also increased notably (P<0.01).Conclusion： Low-dose of tunicamycin caused a certain degree of
endoplasmic reticulum stress, which activated moderate autophagy, decreasing macrophage apoptosis. It may help to reduce the extent of
the atherosclerosis. |
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