| 顾凡磊 赵瑞芳 季志英 吴哈 李益卫.儿童化学发光免疫分析中稀释方法的探讨[J].,2014,14(1):114-116 |
| 儿童化学发光免疫分析中稀释方法的探讨 |
| The Discussion of Dilution Method in Children usingChemiluminescent Immunoassay |
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| DOI： |
| 中文关键词: 稀释 化学发光免疫分析 儿童 |
| 英文关键词: Dilution Chemiluminescent Immunoassay Children |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨儿童化学发光免疫分析中标本量过少时，用怎样的稀释介质以及稀释方法解决这个问题，分析不同的稀释介质 |
| 英文摘要: |
| Objective: To address the problem of undersampling in child chemiluminescene immunoassay by investigating the
influence of dilution mediumand method on detection of serumtriiodothyronine(Total T3) concentration. Method:Serumtriiodothyronine
(Total T3) concentration, a routinely screened indicator in children physical examinations, was tested. Serum samples were collected
from 30 children. UniCel DXI800 appliance produced by Beckman Coulter Inc was used to test the concentration of serum
triiodothyronine as diluted two and four times by Access Total T3 Calibrators S0 (TT3S0), 0.9 %sodium chloride injection, and sterile
water for injection respectively. By comparing the concentration test results before and after dilution, the matrix effect on test results was
investigated. Based on the experimental results, the workable diluents and dilution multiples can be identified to be applied in medical
tests.Results: Paired t-tests between before-and after-dilution groups showed that the difference is statistically insignificant in samples
diluted by TT3S0 two times (t=0.7937, P>0.05), TT3S0 four times dilution (t= -0.2503, P>0.05), and sterile water two times dilution (t=
-0.2845, P>0.05). The difference is statistically significant in samples diluted by 0.9 %sodium chloride injection two times (t= -6.4686,
P<0.05), 0.9 %sodium chloride injection four times (t= -3.9842, P<0.05) and sterile water four times (t= -1.9957, P<0.05). Conclusions: TT3S0 two times and four times dilution, as well as sterile water two times dilution can work as satisfactory dilution method.TT3S0 can
be diluted 2 or 4 times to be applied as dilution medium. When it is difficult to get access to the above dilution medium, sterile water two
times dilution can also be used. |
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