| 胡晓丽 王雪峰 张亮 庞伟 李楠.新生小鼠缺氧缺血性脑损伤模型的制作研究[J].,2014,14(1):57-61 |
| 新生小鼠缺氧缺血性脑损伤模型的制作研究 |
| The Production Research of Neonatal Mice Modelof Hypoxic-Ischemic Brain Damage |
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| DOI： |
| 中文关键词: 缺氧缺血性脑损伤 动物模型 小鼠 病理 |
| 英文关键词: Hypoxic ischemic brain damage Animal model Mice Pathology |
| 基金项目:辽宁省教育厅基金项目（L2012336） |
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| 中文摘要: |
| 目的：通过对动物模型的制作模拟新生儿围产期缺氧缺血性脑损伤，研究其脑组织病理变化，为新生儿缺氧缺血性脑损
伤的病理生理的研究以及进一步有效的治疗提供实验基础。方法：将40 只7 d新生昆明小鼠分四组，分别为正常组（A 组）、单
侧颈总动脉结扎组（B 组）、单侧颈总动脉结扎＋缺氧组（C 组）和双侧颈总动脉结扎组（D 组）。单侧颈总动脉结扎组（B 组）行
右侧颈总动脉结扎；单侧颈总动脉结扎＋缺氧组（C 组）行右侧颈总动脉结扎后将其置于20 ℃的恒温50 mL密闭容器中，分不
同的时间将其取出；双侧颈总动脉结扎组（D 组）行双侧颈总动脉结扎，各组术后均送回母鼠身边继续母乳喂养，三天后再作病
理检测。结果：行单侧颈总动脉结扎加缺氧60 min 时, 小鼠结扎侧皮质及海马区出现病理改变，随着缺氧时间延长（90 min、
100 min、120 min）病变范围逐渐扩大，病理改变越明显。结论：本实验显示单侧颈总动脉结扎同时缺氧一定时间可以导致小鼠
脑组织损伤，脑细胞发生病理改变，且皮层及海马区域的神经细胞对缺氧缺血最为敏感, 从而为进一步研究新生儿缺氧缺血性
脑损伤提供了较为可靠的模型。 |
| 英文摘要: |
| Objective: To investigate the pathological changes of brain tissue by building he animal model simulated neonatal
perinatal hypoxic ischemic brain damage, and study neonatal hypoxic ischemic brain injury in the pathophysiology to provide an
experimental basis for further effective treatment.Methods: 40 postnatal 7 d neonatal Kunming mice were divided into four groups:
normal group( A group), unilateral common carotid artery ligation group ( group B), unilateral common carotid artery ligation plus hypoxia
group ( group C) and bilateral common carotid artery ligation group ( D group). Unilateral carotid artery ligation group ( group
B) underwent right carotid artery ligation; Unilateral common carotid artery ligation plus hypoxia group( group C) underwent right carotid
artery ligation and put them into the 50 mL container in the constant temperature of 20℃ and the mice taken out at different time.
Bilateral common carotid artery ligation group ( D group ) underwent bilateral carotid artery ligation, and each group after operation were
returned to the maternal side to continue breastfeeding, three days later for histologic detection.Results:Unilateral common carotid
artery ligation plus hypoxia 60min, cortex and hippocampus ligation side in mice brain appeared pathological changes, with prolonged
hypoxia ( 90 min, 100 min, 120 min ) lesions scope gradually expanded, more obvious pathological changes.Conclusion:Unilateral
carotid artery ligation and hypoxia for a certain period of time can cause mice brain injury, brain cells occur pathological changes, and
cortical and hippocampal region of the neural cells to hypoxia and ischemia are the most sensitive, so as to further study of neonatal
hypoxic-ischemic brain injury provides a more reliable model. |
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