| 赵建法陈升东于苏文乔莉霞崔红霞.NGF 对大鼠胚胎中脑神经细胞体外增殖和分化的影响[J].,2012,12(26):5045-5048 |
| NGF 对大鼠胚胎中脑神经细胞体外增殖和分化的影响 |
| The Effects of NGF on Proliferation and Differentiationof Rat Midbrain Nerve Cell in Vitro |
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| DOI： |
| 中文关键词: 神经生长因子 增殖 分化 中脑神经细胞 胚胎大鼠 |
| 英文关键词: NGF Proliferation Differentiation Midbrain nerve cell Embryo rat |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨神经生长因子(nerve growth factor, NGF)对大鼠胚胎中脑神经细胞体外增殖和分化的影响。方法：在体外分离培养
大鼠胚胎中脑神经细胞的培养液中加入不同浓度(10、50、100、200ng/ml)的NGF，培养不同时间，以不加神经营养因子的细胞为对
照组，通过MTT 法检测细胞活性，神经元特异性烯醇化酶免疫细胞荧光技术鉴定神经细胞，光镜下形态学观察各组大鼠中脑神
经细胞体外增殖和分化情况。结果：胚胎中脑神经细胞胞体增大、突起延长且有丰富的神经纤维连结成网络状, 细胞集落数增加,
显示出剂量-效应关系。结论：一定剂量的NGF能促进大鼠中脑神经细胞分化和增殖, 增强其活性。 |
| 英文摘要: |
| Objective: To compare the effects of nerve growth factor (NGF) on the proliferation and differentiation of rat midbrain
nerve cell in vitro. Method: The culturemedium of midbrain nerve cell swas added NGF group or withoutany neurotropic factors (controlgroup),
The NGF different concentration for 10, 50, 100, 200 ng/ml, in different time to train nerve cells. The proliferation and differentiation
of midbrain nerve cell were observed by convert microscope and MTT detection cell activity detected and the Neurons were
identified by neuron-specific enolase immunofluorescence technic. Results: The cultured embryonic nerve cell body were aggrandized,
the cell protuberance was prolonged, plenty of neuron fiber formed net work, the number of survival spinal cord neurons, which showed
the dose- effect relation. Conclusion: Certain concentration of NGF(50.0~100.0ng/ml) can promote the differentiation and proliferation of
embryonic nerve cell , and enhance the activity of the neurons. |
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