| 吴楠1 刘薇2 卢光琇2△.人外周血树突状细胞体外诱导扩增的研究[J].,2012,12(25):4948-4949 |
| 人外周血树突状细胞体外诱导扩增的研究 |
| Experimental Study on Induction and Expansion of Dendritic Cells fromHuman Peripheral Blood In Vitro |
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| DOI： |
| 中文关键词: 树突状细胞 细胞培养 细胞因子 |
| 英文关键词: Dendritic cell Cell Culture Cytokine |
| 基金项目: |
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| 摘要点击次数: 620 |
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| 中文摘要: |
| 目的：探讨可用于临床治疗功能成熟的DC 体外扩增的优化培养方案。方法：胎牛血清培养基联合细胞因子rhGM-CSF(100
ng/mL)和rhIL-4(50 ng/mL)扩增人外周血分离的单个核细胞，细胞培养分别按5×106/mL、6×106/mL和7×106/mL的密度，加入6
孔培养板。第6d 加入rhTNF-a(100 ng/mL)联合培养，分别于第6 d，第9 d 和12 d 收获细胞。从形态学、细胞表面标志方面进行鉴
定。结果：显微镜观察，经过9 d 诱导后, 培养细胞具有典型树突细胞外形。流式细胞仪分析，6×106/mL密度的细胞培养组培养到
第9 天最宜。结论：细胞具有典型的DC 的形态特征，细胞表型及功能实验证实其DC 的特性，说明建立的血清培养基联合细胞因
子rhGM-CSF、rhIL-4 和rhTNF-a 体外诱导DC 的方法是切实可行的。 |
| 英文摘要: |
| Objective: To investigate a culture system for DC expansion. Methods: Using serum medium with rhGM-CSF
(100ng/mL) and rhIL-4 (50ng/mL) for 6 days, rhTNF-a (100ng/mL) was added on the 6th day of culture. Human peripheral blood were
seeding at the concentration of 5×106/mL, 6×106/mL and 7×106/mL in 6-well flat-bottomed plates. Suspension cells were harvested at
days 6, 9 and 12 and analysed on morphology, phenotype observed. Result: Cells on day 9 of culture displayed DC-like morphology.
Phenotype analysis on DCs acquired showed that the cells of 6×106/mL cultured for 9 days contained a higher percentage of DC cells. |
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