| 陈建新赵玉石成军卢红光李野.烟雾加气管内滴入PPE 诱发非均质性肺气肿模型[J].,2012,12(25):4837-4840 |
| 烟雾加气管内滴入PPE 诱发非均质性肺气肿模型 |
| Induction of Heterogenous Emphysema by Cigarette Inhalation and ElastaseTracheal Instillation |
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| DOI： |
| 中文关键词: 兔 肺气肿 香烟 弹性蛋白酶 疾病模型 |
| 英文关键词: Rabbit Emphysema Smog Elastase Disease models |
| 基金项目: |
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| 摘要点击次数: 811 |
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| 中文摘要: |
| 目的：探讨烟雾与弹性蛋白酶（PPE）联合应用在兔阻塞性肺气肿模型形成过程中对肺组织X 线、动脉血气、肺组织形态学结
构的影响以及如何在较短时间内诱发出类似于人类疾病的非均质性肺气肿模型。方法：将40 只小白兔随机分为吸烟组、注酶组、
联合组及对照组4 组，分别给予单纯香烟熏吸、气管内注入猪胰弹性蛋白酶、烟熏加气管内注入弹性蛋白酶及单纯气管内注入生
理盐水作为对照组。7 周后分别进行肺组织X 线、动脉血气和肺组织形态学检查。结果：注酶组和联合组的平均肺泡数(Na)、肺泡
隔面密度(Ds)及PaO2 减少，PaCO2、肺总容积(TLC)和肺泡直径(Da)增加，与对照组比较，差异有显著性(P< 0.05)。注酶组和联合组
大鼠平均肺泡数(MAN)与正常对照组大鼠相比明显减少(P<0.05)，平均肺泡面积（MAA）、平均内衬间隔（Lm）明显大于正常对照
组(P<0.05)，而吸烟组与正常对照组无统计学差异(P>0.05)。结论：烟雾可强化弹性蛋白酶在诱发肺气肿模型过程中的作用,二者联
合应用可加快兔肺气肿模型的诱导过程。 |
| 英文摘要: |
| Objective: To investigate the effects of smog and elastase on the pulmonary functions and histology during the
pathogeny of rabbits' chronic obstructive pulmonary emphysema and to induce the pulmonary model which is similar to the human disease.
Methods: Forty rabbits were divided into four group: smoking, elastase, elastase combined smoking and control groupand treated respectively
with smoking only, inject elastase into trachea, elastase combined smoking and inject normal saline respectively. Seven weeks
later, pulmonary-X, artery blood gas analysis and pulmonary histology were detected. Results: Compared with the control group, in both
elastase and combined groups, density of alveolar septum (Ds), number of alveolus (Na) and PaO2 decreased, PaCO2, total lung capacity
(TLC) and diameter of alveolus(Da) increased on the contrary (P<0.05), while in both elastase and combined groups, Mean alveolar number
(MAN) decreased and Mean alveolar area (MAA), Meanlinear intercept,Lm (Lm) increased (P<0.05), but no change happened in
smoking group (P>0.05). Conclusions: Rabbits obstructive pulmonary emphysema can be induced by elastase. |
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