| 李晓辉张维东△ 王兆朋张月英贾青王朝霞.蝎毒多肽提取物对卵巢癌SKOV3 细胞HPA 与VEGF 抑制作用
的实验研究[J].,2012,12(24):4624-4626 |
| 蝎毒多肽提取物对卵巢癌SKOV3 细胞HPA 与VEGF 抑制作用
的实验研究 |
| Inhibitive Effect of Polypeptide Extract from Scorpion Venom (PESV) onthe Expression of HPA and VEGF in SKOV3 Cell Lines |
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| DOI： |
| 中文关键词: 蝎毒多肽提取物 卵巢癌 HPA VEGF |
| 英文关键词: Polypeptide extract from scorpion venom (PESV) Ovarian cancer Heparanase (HPA) Vascular endothelial growth factor
(VEGF) |
| 基金项目:国家自然科学基金资助项目（81073102；30873408）；山东省自然科学基金资助项目（ZR2010HQ003；2009ZRC03044）；
济南市科学技术发展计划（201004012） |
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| 中文摘要: |
| 目的：研究蝎毒多肽提取物(polypeptide extract from scorpion venom,PESV)对卵巢癌SKOV3 细胞HPA 与VEGF 表达的影
响，进一步探讨其抗血管生成的分子机制。方法：卵巢癌SKOV3 细胞分为对照组、PESV 组，免疫组织化学方法检测各组HPA、
VEGF 的表达，western-blot 方法检测各组HPA 在蛋白水平的表达。结果：与对照组相比，PESV 组HPA、VEGF 表达明显降低（P＜
0.05），进一步检测发现PESV 组HPA 在蛋白水平表达亦明显下调（P＜0.05）。结论：PESV 能够抑制卵巢癌SKOV3 细胞的生长，
其机制可能与抑制HPA、VEGF 的表达有关。 |
| 英文摘要: |
| Objective: To investigate the effect of polypeptide extract from scorpion venom(PESV) on the expression of HPA and
VEGF in SKOV3 cell lines, and to explore the antiangiogenic mechanisms. Methods: SKOV3 cells were divided into control group and
PESV group. Immohistochemistry was performed to determine the expression of HPA and VEGF in each group.Western-blot was used
to detect the protein expression of HPA. Results: Compared with that in the control group, the expression of HPA and VEGF was significantly
downregulated in PESVgroup, and the amount of HPA on protein level reduced significantly. Conclusions: PESV can inhibit the
growth of SKOV3 cells, and the mechanism is probably related with the inhibition of the expression of HPA and VEGF. |
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