| 臧怡1 黄立群1 王玉强2 周毅1 杜子秀1.新型PEI 衍生物在COS-7 细胞中的转染与毒性研究[J].,2012,12(24):4611-4613 |
| 新型PEI 衍生物在COS-7 细胞中的转染与毒性研究 |
| Transfection and Cytotoxicity Studies of Novel PolyethyleneimineDerivative on COS-7* |
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| DOI： |
| 中文关键词: 基因载体 PEI 衍生物 转染效率 细胞毒性 |
| 英文关键词: Gene vector PEI derivative Transfection efficiency Cytotoxicity |
| 基金项目:国家自然科学基金项目(200801198） |
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| 中文摘要: |
| 目的：寻找一种新型的转染效率高，毒性低的非病毒基因载体。方法：通过化学方法合成Polyimine-MPEI，然后以不同质量
比包裹绿色荧光蛋白质粒，检测在COS-7 细胞中的转染效率和毒性。结果：在比例从5 到100 之间，转染效率均比较理想，能达到
1.00E+07 以上，Polyimine-MPEI 的毒性也很小，细胞的生长率均在80%以上，明显高于PEI25KDa 对照组。结论：Polyimine-MPEI
是一个很有研究前景的聚合物载体，具有高转染效率低毒性的特点，可以通过延长反应时间，增加分子量，增大转染能力。 |
| 英文摘要: |
| Objective: To find a novel nonviral gene vector with a high transfection efficiency and low cytotoxicity. Methods:
Polyimine-MPEI is synthesized, then packed with luciferase gene at different mass ratio. The transfection efficiency and cytotoxitity of this
polyplex was detected. Results: Frommass ratio 5 to 100, the transfection efficiency of the polyplex was above 1.00E+07 and it was relatively
ideal. Its cytotoxicity was lower comparing with the PEI25KDa control group and the cell viability was all above the 80%. Conclusions:
Polyimine-MPEI is a polycation vector with a bright research prospect, which has high transfection efficiency and low cytotoxicity. Prolonging
the synthesis time and making its molecular weight higher can increase the transfection efficiency. |
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