| 黄崇友1 臧晓方2△ 熊军2 蔡智2.退变腰椎间盘组织细胞凋亡相关蛋白酶Caspase-10 的表达研究[J].,2012,12(23):4517-4520 |
| 退变腰椎间盘组织细胞凋亡相关蛋白酶Caspase-10 的表达研究 |
| Research on Expression of Apoptosis-related Protease Caspase-10 in LumbarDisc Degeneration |
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| DOI： |
| 中文关键词: 腰椎 椎间盘退变 细胞凋亡 Caspase |
| 英文关键词: Lumbar Disc degeneration Cell apoptosis Caspase |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨凋亡相关蛋白酶Caspase-10 在腰椎间盘退变中的表达。方法：45 例腰椎间盘突出症病人按年龄分为青年、中年和
老年患者组，5 例青少年型特发性脊柱侧弯病人作为对照组。患者组标本均为术中所取出L4/5 新鲜椎间盘髓核组织，对照组标本
均为术中所取出L2/3 新鲜椎间盘髓核组织。采用TUNEL 法和免疫组织化学S-P 法，分别检测腰椎间盘髓核中的凋亡阳性细胞
率及Caspase-10 的表达情况。结果：对照组髓核内TUNEL 阳性细胞率为(17.80±0.62)%。青年、中年、老年患者组髓核内TUNEL
阳性细胞率分别为(45.71±2.05)%、(53.65±2．93)%和(68.39±4.33)%。四个组两两之间差异均有统计学意义( P＜0.05 )。对照组
髓核内Caspase-10 染色阳性细胞率为(32.60 士1.64)%。青年、中年、老年患者组髓核内Caspase-10 染色阳性细胞率分别为(50.67
士2.89)%、(63.12 士4.61)%和(75.28 士4.26)%。四个组两两之间差异均有统计学意义( P＜0.05 )。髓核内Caspase-10 染色阳性细
胞率与TUNEL 阳性细胞率和年龄之间均呈正相关( P＜0.01 )。结论：退行性变腰椎间盘组织中凋亡细胞率以及Caspase-10 的表
达均高于正常椎间盘组织，年龄是细胞凋亡的重要影响因素，细胞凋亡以及Caspase-10 的表达上调可能在腰椎间盘退行性变的
发生和发展中发挥重要作用。 |
| 英文摘要: |
| Objective: To investigate the expression of apoptosis-related protease Caspase-10 in lumbar disc degeneration.
Methods: 45 patients with lumbar disc herniation are divided into young, middle-aged and old patient group. 5 adolescent idiopathic
scoliosis patients act as control. L4/5 fresh nucleus pulposus were removed from the patient group; L2/3 fresh nucleus pulposus were
removed from the control group. The rate of apoptotic cells and the expression of Caspase-10 were detected by TUNEL method and
immunohistochemistry SP method. Results: The rate of TUNEL positive cells in the control group is (17.80 ± 0.62)%. It is (45.71 ±
2.05)% in the youth patient group, (53.65 ± 2.93)% in the middle age patient group and (68.39 ± 4.33)% in the old patient group.
Differences between each of four groups were significant (P＜0.05). The Caspase-10 positive cell rate is (32.60 ± 1.64)% in the control
group, (50.67±2.89)% in the youth patient group, (63.12 ±4.61)% in the middle age patient group and (75.28 ± 4.26)% in the old
patient group. Differences between each of four groups were significant (P ＜0.05). Both the Caspase-10 positive cell rate and
TUNEL-positive cell rate have positive correlation with age (P＜0.01). Conclusions: Apoptosis and the expression of Caspase-10 protein
are higher in the disc degeneration tissues than normal. Age is an important factor of apoptosis. Apoptosis and Caspase-10 may play an
important role in the lumbar intervertebral disc degeneration. |
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