| 陈晨1 王晋2 谢学军2 穆士杰1 张献清1 安群星1.恒磁场抑制缺血缺氧条件下大鼠骨髓间充质干细胞凋亡[J].,2012,12(22):4252-4255 |
| 恒磁场抑制缺血缺氧条件下大鼠骨髓间充质干细胞凋亡 |
| Constant Magnetic Field Inhibit Bone Mesenchymal Stem Cells Apoptosisunder Hypoxia and Serum Deprivation Condition |
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| DOI： |
| 中文关键词: 骨髓间充质干细胞 缺血缺氧 PI3K/Akt 凋亡 恒磁场 |
| 英文关键词: BMSCs Hypoxia and serum deprivation PI3K/Akt Apoptosis Constant Magnetic Fields |
| 基金项目:陕西省社发攻关项目（2010K16-04-04） |
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| 中文摘要: |
| 目的：研究恒磁场对体外缺血缺氧培养条件下大鼠骨髓间充质干细胞（Bone marrow-derived mesenchymal stem cells, BMSCs）
凋亡的影响并探讨其作用机制。方法：采取大鼠骨髓，以密度梯度离心分离出单个核细胞（MNCs），于体外培养并由牛垂体提取物
（PEX）诱导扩增传代培养出骨髓间充质干细胞（MSCs）。经形态学和流式细胞仪检测MSCs 表面标志物鉴定后，将骨髓间充质干
细胞（BMSCs）在缺血缺氧条件下培养，通过TUNEL 检测比较不同组别细胞的凋亡率和蛋白印迹法（western blot）来观察细胞中
特定蛋白质的变化。结果：① 经形态学观察和流式细胞仪检测MSCs 表面标志物鉴定，提示骨髓间充质干细胞培养成功。②缺血
/ 缺氧组与缺血/ 缺氧+ 磁场组比较，缺血缺氧组的凋亡率显著性增加，Akt 磷酸化水平显著上升(P<0.05)。提示恒磁场可以使
PI3K(Phosphoinositide-3kinase)/Akt(ProteinkinaseB,PKB)信号通路被激活而抑制凋亡的发生。结论：恒磁场通过激活PI3K/Akt 信号
通路抑制体外缺血缺氧条件下培养的骨髓间充质干细胞的凋亡。 |
| 英文摘要: |
| Objective: To investigate the influence of constant magnetic fields on the apoptosis of marrow-derived mesenchymal
stem cells under hypoxia and serum deprivation condition and further to explore its mechanism. Methods:Mononuclear cells were
isolated from rat bone marrow by density-gradient centrifugation and then they were cultured on fibronectin-coated plates supplied with
bovine pituitary extract to get MSCs. BMSCs were identified by Morphology and Surface molecule marker analyzed by FCM. Then the
marrow-derived mesenchymal stem cells were cultured under hypoxia and serum deprivation condition (hypoxia and SD), the proportion
of apoptosis and the level of p-Akt in different groups were detected by tunnel analysis and Western bolt respectively. Results: ① The
marrow-derived mesenchymal stem cells were successfully obtained by identification of morphology and the MSCs specific surface
marker. ② The proportion of apoptosis is significantly higher in the group under hypoxia and SD than that of group under constant
magnetic fields plus hypoxia and SD. The level of p-Akt upgraded significantly, therefore indicated the activation of PI3K/Akt pathway
(P<0.05). Conclusion:Constant magnetic field inhibited the apoptosis of marrow-derived mesenchymal stem cells under hypoxia and SD
by activating PI3K/Akt. |
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