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目的：通过测量3 种不同浓度龙胆紫溶液浸入充填体边缘的深度，比较三者在充填体微渗漏检测实验中的性能。方法：将因正畸治疗拔除的人离体前磨牙30 颗随机分为A、B、C3 组，每组10 颗。于离体牙颊面釉牙骨质界冠方1mm 处制备4mm×3mm× 2mm 的标准V 类洞型。常规树脂充填并经冷热循环（5℃/55℃，400 次）后分别放入浓度为0.5%（A 组）、1%（B 组）、2%（C 组）的龙胆紫溶液中浸泡96h。三用枪冲洗吹干后将离体牙沿颊舌向垂直于充填体表面片切。在根管显微镜下观察离体牙充填体边缘染料浸入情况并摄片。采用Image-Pro Plus 6.0 图像分析软件测量龙胆紫溶液浸入深度并记录。结果：A、B、C3 组龙胆紫溶液渗入深度分别为(0.59±0.22)mm、(1.38±0.32)mm、(1.52±0.45)mm，3 组结果之间有统计学差异（F= 21.431，P＜0.05）。其中A、B 组有统计学差异（t= 5.138，P＜0.05），A、C 组有统计学差异（t= 6.082，P＜0.05），B、C 组无统计学差异（t= 0.944，P＞0.05）。结论：2%、1% 龙胆紫溶液渗透速度较快，0.5%龙胆紫溶液渗透速度最慢；0.5%龙胆紫溶液组渗透稳定性较好，1%龙胆紫溶液次之，2%龙胆紫溶液渗透稳定性最差。

英文摘要:

Objective: To investigate the performance of gentian violet solution on three kinds of concentration in the microleakage detection experiments of obturator by detecting the depth of their penetration in the edge of obturator. Methods: Thirty recently extracted human second premolars teeth were chosen and standardized mixed Class V cavities (4 mm ×3 mm and 2 mm deep[1,2) were cut on the buccal surfaces with the gingival margins placed 1.0 mm above the cemento-enamel junction (CEJ). The teeth were randomly assigned into 3 groups (A/B/C, n=10): Conventional resinous fillings. After keeping the specimens for 24 hours at 37 degrees C and 100% humidity, they were thermocycled for 400 cycles at 5℃and 55℃, sealed with nail varnish except 1 mm beyond the margins of restoration twice, and then separately immersed in different concentrational gentian violet basic for 96 hours (0.5%, A;1%, B;2%, C). Samples were then sectioned mesiodistally and viewed at 40× magnification under a root canal microscope for leakage at the gingival margin and taken pictures. The microleakage was measured with the image analysis software Image-Pro Plus 6.0 and the data were recorded in data of dye penetration to the whole path from the cavosurface margin of the proximal boxes to the end of the post. Analysis of variance and the T-test were used to evaluate the data (P=0.05). Results: The depth of the penetration of gentian violet solution of the three groups of teeth separately were A (0.59±0.22)mm, B (1.38±0.32)mm, C (1.52±0.45)mm. There were significant differences among the three groups in the depth of the penetration of gentian violet solution （F=21.431，P＜0.05）. And there were significant differences between A and B （t=5.138，P＜0.05）, also between A and C （t=6.082，P＜0.05）. But not between B and C （t=0.944，P＞ 0.05）. Conclusions: With regards to microleakage, the penetration speed of gentian violet solution of 1% and 2% was the biggest. That of 0.5% is a little smaller. The penetration stability of gentian violet solution of 0.5% was the best, and that of 1% was worse, while that of 2% was the worst.

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